Research Specialist Mass Spectrometry

About the Role:

We areseekinga highly skilled and motivated Research Specialist with advancedexpertisein mass spectrometry-based data analysis particularly in quantitative label-free and/or stable isotope labeling proteomics data analysis global and targeted metabolomics andlipidomics MS imaging as well as crosslinking mass spectrometry (XL-MS). The successful candidate will playa central rolein the computational and analytical components of research projects applystate-of-the-artbioinformatics tools quantitative analysis strategies and multi-omics data-integration approaches to support a wide range of biological research programs. Although this is a data-focused role wet-labproficiencyincluding sample handling operation and method setup on OrbitrapTribridinstrument are highly valued and considered a strong asset.

AbouttheMass Spectrometry Department:

The mass spectrometry department at HHMIJaneliaResearch Campus is a new shared resourcefounded in 2024which is dedicated to advancing scientific research and innovation in the field of proteomics metabolomicslipidomicsand many more. We are equipped withpioneeringinstruments and solutions tofacilitatethe study of the dynamics structures functions and interactions of various biomolecules which may correlate to cellular dynamics neural and behavioral mechanisms.

What we provide:

• A competitive compensation package with comprehensive health and welfare benefits.

• A supportive trainingenvironmentwith opportunities toparticipatein scientific workshops and conferences.

• Collaborative and innovative work environmentwith opportunitiesto make a meaningful impact on scientific research and discovery.

• Amenities that enhance work-life balance such as on-site childcare free gyms available on-campus housing social and dining spaces and convenient shuttle bus service to Janelia from the Washington D.C. metro area.

Whatyoulldo:

• Working under the direct supervision of the Department Director you will serve as a data-analysis specialist with growing independence in designing executinginterpretingand visualizing complex mass-spectrometrybased experiments.

• Lead and support in-depth analysis of DIA and DDA proteomic datasets TMT-based multiplexed proteomics sets global and targeted metabolomicslipidomics XL-MS as well as MS MALDI imaging datasets usingstate-of-the-artbioinformatics and statistical tools.

• Collaborate closely with researchers to guide study design interpret results troubleshoot challenging datasets and contribute to the development and optimization of new mass spectrometry methodologies and workflows.

• Developoptimize andvalidatecomputational workflows and data-analysis pipelines including QC assessment normalization strategies spectral library generation PTM analysis peptide/protein quantification metabolite identification lipid species annotation and crosslink identification.

• Integrate multi-omics datasets whenappropriateand contribute to biological interpretation in collaboration with project teams.

• Keepaccurate detailed electronic records of experimental methods raw and processed data computational workflows andanalysisoutcomes.

• Help support day-to-day operations of the mass-spectrometry facility includingmaintaininglaboratory workstations reporting instrument issues and ensuring workflows meet high research-quality standards.

• Train junior personnel or visiting researchers in data-analysis tools interpretation strategies and laboratory best practices.

• Support the departments commitment to a collaborativeequitable and inclusive work environment.

What you bring:

• Masters or Ph.D. degree in computing science computational biology biochemistry biology chemistry or a closely related field.

• At least 4 years (BS) or 2 years (MS) of experience in analysis andvisualizationof proteomics metabolomicslipidomics MS imaging or XL-MS data.

• Demonstratedexpertisein deep data analysis of: DIA proteomics (e.g. DIA-NNSpectronaut Skyline) TMT-multiplexed proteomics (e.g.MaxQuant Proteome Discoverer) Metabolomics andlipidomicsdatasets (e.g. Compound DiscovererLipidSearch MS-Dial) MS imaging (e.g.SCiLS) Crosslinking MS data (e.g.XlinkXMeroX)

• Strong computational aptitude and familiarity with statistics data visualization and large-scale datamanagement;experience using R or Python is a plus.

• Proficiencywith mass-spectrometeroperatingand tuning software; experience withThermoXcaliburand OrbitrapTribridplatforms is highly preferred.

• Enthusiastic self-motivated and able to work both independently and within a collaborative research environment.

• Excellent organization communication and time-management skills.

• Strong attention to detail especially in documentation QC tracking and method validation and reproducibility.

• Ability to work effectively as part of a diverse multidisciplinary team.

Physical Requirements

Remaining in a normal seated or standing position for extended periods of time; reaching and grasping by extending hand(s) or arm(s); dexterity to manipulate objects with fingers for example using a keyboard; communication skills using the spoken word; ability to see and hear within normal parameters; ability to move about workspace. The position requires mobility including the ability to move materials weighing up to several pounds (such as a laptop computer or tablet). Work may involve possible exposure to malodorous vapors low dose radiation contamination by toxic chemicals and acids and presence of carcinogenic substances or other hazardous materials.

Persons with disabilities may be able to perform the essential duties of this position with reasonable accommodation. Requests for reasonable accommodation will be evaluated on an individual basis.

Please Note:

This job description sets forth the jobs principal duties responsibilities and requirements; it should not be construed as an exhaustive statement however. Unless they begin with the word may the Essential Duties and Responsibilities described above are essential functions of the job as defined by the Americans with Disabilities Act.

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