Research Assistant, Biochemistry, Kuriyan
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Job Location:
Nashville, IN - USA
Monthly Salary:
Not Disclosed
Posted on:
5 days ago
Vacancies:
1 Vacancy
Job Summary
The Kuriyan lab studies the structural mechanisms of the protein switches that carry out cellular signal transduction. We use biochemical biophysical structural and cell biological analyses to elucidate molecular mechanisms of how proteins function and also study how these mechanisms change with evolution. Aberrant signaling from these molecular switches underlies the onset of many human diseases including several types of cancers. The Kuriyan lab is actively pursuing innovative approaches to modulate the functions of these targets and to advance the development of better therapeutics.
Duties and Responsibilities:
The individual is expected to support research activities that include but are not limited to molecular cloning cell culture protein biochemistry and cellular analysis of protein function.
The ideal candidate would have prior laboratory experience working in molecular biology cell biology biochemistry or related fields. The ability to operate independently as well as part of a team is critical.
Preferred experience and skills:
Preparation of competent cells LB medium and agar plates
Aseptic technique for handling bacteria and mammalian cell culturing
Molecular cloning to prepare various constructs and variants of the proteins of interest with suitable vectors for recombinant protein expression or cellular analysis
Maintenance and subculturing of insect cell and mammalian cell lines
Recombinant protein expression using insect cells or mammalian cells.
Protein purification using centrifugation affinity chromatography ion-exchange and size-exclusion chromatography
Expression purification and in vitro reconstitution of integral membrane proteins
Biochemical and biophysical assay development to measure protein activity protein-protein and protein-small molecule interactions
General duties in laboratory research and development:
Provide general support for everyday lab maintenance tasks
Participate in laboratory meetings and planning sessions
Collect record organize and verify the accuracy of experimental data
Qualifications
A Bachelors degree in molecular biology biochemistry cell biology biological sciences chemistry or a related field from an accredited institution of higher education is necessary.
2 or more years experience in biomedical research is necessary.
Experiencewithorganizationinventoryandrecordkeepingispreferred
Excellentorganizationalandcommunicationskills(verbalandwritten)arepreferred
Key Skills
- Laboratory Experience
- Spss
- Data Collection
- DNA Isolation
- Stata
- Biochemistry
- Qualitative Research Interviewing
- Research Experience
- Statistical Software
- Molecular Biology
- Research Laboratory Experience
- Western Blot
About Company
At Vanderbilt University , our work - regardless of title or role - is in service to an important and noble mission in which every member of our community serves in advancing knowledge and transforming lives on a daily basis. Located in Nashville , Tennessee, on a 330+ acre campus and ... View more
