Associate Scientist - Flow Cytometry
Associate Scientist - Flow Cytometry
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$ 99705 - 129030
1 Vacancy
Job Description
Were here for one reason and one reason only to cure cancer. Every moment is dedicated to developing treatments and every action moves us one step closer to our goal. Weve made incredible scientific breakthroughs and our pioneering personalized CAR Tcell therapies have changed the paradigm. But were not finished yet.
Join Kite as we make even bigger advances in cancer therapies and help shape where our business and medical science goes next.
We believe every employee deserves a great leader. People Leaders are the cornerstone to the employee experience at Gilead and Kite. As a people leader now or in the future you are the key driver in evolving our culture and creating an environment where every employee feels included developed and empowered to fulfil their aspirations. Join Kite and help create more tomorrows.
Job Description
We are seeking an experienced creative and motivated Associate Scientist to join our growing team in Santa Monica. In this role you will contribute to the companys efforts to develop nextgeneration cell therapies in solid tumor malignancies. The successful candidate will have extensive experience in flow cytometry cell sorting tissue culture and sterile technique and in maintenance and tracking of laboratory inventory. Experience in a flow cytometry core lab is highly desirable. In addition the candidate will be expected to work in a collaborative crossfunctional team environment perform experiments organize and analyze results communicate effectively and present findings in team meetings.
Duties And Responsibilities
Seeking a highly motivated individual who shares an enthusiasm for flow cytometry cell biology and loves to learn. Prior experience with flow cytometry instruments required.
General responsibilities include but are not limited to:
- Performance of daily QC maintenance and troubleshooting of flow cytometry instruments (BD FACSCanto BD LSRFortessa BD FACSymphony A5 SE Invitrogen Attune NxT Cytek Aurora) and high throughtput systems (BD HTS Invitrogen CytKick Max). Experience in cell sorting is a plus. (BD FACSAria III Fusion Invitrogen Bigfoot).
- Acquisition and analysis of cytometric data using instrument (BD FACSDiva Invitrogen Attune BigFoot Cytek Aurora) and analysis software packages (FloJo OMIQ). Use of highdimensional data analysis techniques and statistical methods for visualization of complex flow cytometry data.
- Familiarity with other software is a plus (FCS Express Invitrogen Attune Cytometric Software Invitrogen SpectroFlo Benchling (ELN and LIMS) GraphPad Prism Microsoft 365 (Word Excel PowerPoint Outlook Teams).
- Development of multicolor reagent panels for conventional and spectral flow cytometers. This includes instrument optimization and antibody titration.
- Evaluation of new cytometry technology (software and hardware) and current literature relevant to flow cytometry keeping the lab up to date.
- Cell engineering to include: cell line maintenance using aseptic technique genetic manipulation of cells according to established molecular biology protocols and creation of cell banks for freezing and/or shipping. Develop characterize and bank/maintain tumor cell lines for use in in vivo and in vitro studies. This involves novel engineering technology and creative problem solving.
- Maintenance of common critical reagent stocks by filtering aliquoting and/or refilling carboys and other highuse containers. Initiating and coordinating instrument vendor service calls and preventative maintenance visits. Ordering and stocking lab supplies.
- Training flow core users on cytometry protocols instruments and software.
- Assisting with automation of flow cytometric assays.
- Collecting organizing interpreting and presenting data in small and large group meetings. Presentations include crosssite research team updates and occasional symposia and user training related to flow cytometry and cell engineering. Creation and presentation of research work at both internal and external poster sessions is required.
- Contribute to a dynamic exciting culture that embraces teamwork and innovation.
Education And Experience - A.S Degree in biologyrelated field with 5 years of experience OR
- B.S Degree in biologyrelated field with 4 years of experience OR
- M.S Degree in biologyrelated field with 2 years of experience OR
- High School Deploma in biologyrelated field with 6 years of experience
Preferred Technical Skills - Degree in biologyrelated field molecular biology immunology or cell biology preferred.
- Prior experience with flow cytometry. Experience in a shared research core lab is desirable.
- Experience with sterile technique and mammalian cell culture.
- Single cell and bulk cell sorting.
- Proficiency in the use of software to support data analysis such as FlowJo FACSDiva GraphPad Prism.
- Strong organizational and timemanagement skills.
- Thorough record keeping skills (documentation and electronic lab notebook).
- Strong ability to troubleshoot independently and to provide highquality results through critical analysis and interpretation. Excellent interpersonal verbal and written communication skills.
- Proven ability to work and think independently and creatively; be an affable and energetic colleague and team player.
For additional benefits information visit:
Gilead Sciences Inc. is committed to providing equal employment opportunities to all employees and applicants for employment and is dedicated to fostering an inclusive work environment comprised of diverse perspectives backgrounds and experiences. Employment decisions regarding recruitment and selection will be made without discrimination based on race color religion national origin sex age sexual orientation physical or mental disabilitygenetic information or characteristic gender identity and expression veteran status or other nonjob related characteristics or other prohibited grounds specified in applicable federal state and local laws. In order to ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973 the Vietnam Era Veterans Readjustment Act of 1974 and Title I of the Americans with Disabilities Act of 1990 applicants who require accommodation in the job application process may contact for assistance. NOTICE: EMPLOYEE POLYGRAPH PROTECTION ACT PAY TRANSPARENCY NONDISCRIMINATION PROVISION Our environment respects individual differences and recognizes each employee as an integral member of our company. Our workforce reflects these values and celebrates the individuals who make up our growing team. Gilead provides a work environment free of harassment and prohibited conduct. We promote and support individual differences and diversity of thoughts and opinion. Please apply via the Internal Career Opportunities portal in Workday.
Kite is a biopharmaceutical company engaged in the development of innovative cancer immunotherapies with a goal of providing rapid longterm durable response and eliminating the burden of chronic care. The company is focused on chimeric antigen receptor (CAR) and T cell receptor (TCR) engineered cell therapies designed to empower the immune systems ability to recognize and tumors. Kite is based in Santa Monica CA. For more information on Kite please visit . Sign up to follow @KitePharma on Twitter at jobs in the United States:
For more information about equal employment opportunity protections please view theKnow Your Rights poster.
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Required Experience:
IC
Employment Type
Full-Time
