Hani Hosseini Far

Hani Hosseini Far

Research Scientist / iPSC Laboratory Manager
Australia
Persian (Farsi), English
Full-time On-site

نبذة عني

Stem-cell and molecular biologist with a PhD (University of Melbourne, 2019) and extensive hands-on experience generating, maintaining, genetically engineering and differentiating human iPSCs. Current iPSC Laboratory Man…

الخبرة

Research Scientist / iPSC Laboratory Manager

Hudson Institute of Medical Research · Melbourne, Australia
Apr 2025 - حتى الآن · 1 سنة 6 أشهر

<p>Manage human iPSC culture, QC, differentiation and research workflows across collaborative projects.</p><p> Develop neuronal-lineage and immune-cell differentiation protocols, including culture-surface optimisation and cellular characterisation.</p><p> Plan CRISPR and prime-editing studies, derive single-cell clones and undertake molecular screening and quality control.</p><p> Train researchers, troubleshoot complex cultures and coordinate documentation and experimental reproducibility.</p>

Postdoctoral Researcher

Hudson Institute of Medical Research · Melbourne, Australia
Feb 2020 - Apr 2025 · 5 سنوات 2 أشهر

<p>Contributed to intestinal organoid and advanced 3D culture projects, including tissue modelling and cell phenotyping.</p><p> Established iPSC-derived macrophage and dendritic-cell systems for interferon and host–pathogen studies.</p><p> Generated gene-edited iPSC models and analysed molecular, imaging and functional assay readouts.</p><p> Collaborated with researchers across molecular biology, genomics and translational science.</p>

R&D Scientist (part-time)

RNAte · Melbourne, Australia
Jan 2023 - Jan 2025 · 2 سنوات

<p>Contributed to iPS cell-based assays and research related to RNA therapeutics and inflammatory responses.</p>

المهارات

Human pluripotent stem cells Sendai reprogramming Episomal reprogramming iPSC derivation Cell expansion Quality control Cryopreservation Single-cell cloning 2D differentiation 3D differentiation Embryoid bodies Neuronal differentiation Organoid culture Hydrogels Matrigel Laminin Poly-L-ornithine Poly-D-lysine CRISPR/Cas9 Gene knockout Gene correction Isogenic disease models PCR genotyping Clone QC Prime editing DNA extraction RNA extraction Reverse transcription PCR RT-qPCR Cloning Western blot Immunostaining Confocal microscopy Fluorescence microscopy Incucyte Flow cytometry FlowJo ELISA Bulk RNA-seq GraphPad Prism SnapGene Python Scientific data analysis SOPs Manuscript preparation Cross-functional collaboration
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