Scientist – Discovery Biology T-cells
Job Location:
San Diego, CA - USA
Salary:
Posted:
Application Deadline:
Vacancies:
Job Summary
Our client a world leader in life sciences and biotechnology is looking for a Scientist Discovery Biology/ T cells based in San Diego CA.
Job Duration: Long Term Contract (Possibility of Extension)
Pay Rate: $49.20/hr on W2
Company Benefits: Medical Paid Sick leave 401K
Join the Discovery Biology team to support a high-priority binder screening campaign for T-cell-based therapies. Characterize candidate binders (scFvs/VHHs) for potency specificity affinity functionality and safety through T-cell engineering and functional validation assays.
Key Responsibilities
- Perform non-viral engineering of primary human T cells using mRNA or transposon-based DNA systems via electroporation/nucleofection; monitor cell viability recovery and synthetic receptor expression.
- Isolate maintain and expand primary T cells from PBMCs and Jurkat cell lines using aseptic techniques.
- Execute high-throughput binder screening and multi-color flow cytometry (FACS) to assess surface expression and phenotypic markers; analyze data using FlowJo.
- Conduct T-cell activation and functional assays including cytotoxicity assays (IncuCyte live-cell imaging/LDH release) cytokine profiling (ELISA/MSD/Luminex) and proliferation assays (CFSE).
- Record protocols deviations raw data and experimental results accurately in an Electronic Lab Notebook (ELN) to support data integrity and regulatory filings.
- Support screening scale-up using liquid-handling robotics as applicable.
Required Qualifications
- Bachelors or Masters degree in Immunology Molecular Biology Bioengineering or a related biological science.
- Minimum 2 years of hands-on wet-lab experience preferably in an industry setting.
- Strong aseptic technique and experience isolating primary T cells from PBMCs.
- Mandatory expertise in flow cytometry acquisition and data analysis.
Preferred Qualifications
- Hands-on experience with non-viral T-cell transfection/electroporation or nucleofection.
- Experience with liquid-handling robotics and high-throughput screening workflows.
- Proficiency in FlowJo and functional T-cell assay techniques.
If interested please send us your updated resume at
Required Skills:
(primary T cells OR human T cells OR T-cell engineering) AND (flow cytometry OR FACS OR FlowJo) AND (electroporation OR nucleofection OR transfection OR mRNA delivery OR transposon)
Required Education:
Bachelors
