Research Assistant I Laboratory of Dr. Ron Evans

The Research Assistant I will support NIH R01-funded research focused on human induced pluripotent stem cell (iPSC) differentiation and beta cell transplantation for the development of novel cell replacement therapies for diabetes. Working under the direction of the Principal Investigator and in collaboration with postdoctoral fellows staff scientists and research collaborators the incumbent will assist with maintaining human iPSC cultures performing multistage pancreatic and beta cell differentiation protocols characterizing differentiated cells and supporting transplantation studies in mouse models of diabetes.

The successful candidate will be responsible for following established laboratory protocols maintaining accurate and detailed experimental records and ensuring consistent application of aseptic techniques and quality standards. This position requires strong organizational skills attention to detail and the ability to execute complex time-sensitive procedures with accuracy and reproducibility. The Research Assistant I will have the opportunity to develop technical expertise in stem cell biology molecular and cellular biology techniques genome engineering and small-animal research while contributing to a collaborative and multidisciplinary research environment.

Who We Are

The Salk Institute is an internationally renowned research institution that valuesall members of our scientific community. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces perspectives across discipline professional acumen and unique life experiences fostering innovation and a sense of belonging. Together we strive to improve the wellbeing of humanity through groundbreaking research.

Founded by Jonas Salk developer of the first safe and effective polio vaccine the Institute is an independent nonprofit research organization and architectural landmark: small by choice intimate by nature and fearless in the face of any challenge. Salks vibrant community has many talented individuals from varied backgrounds each playing a crucial role in driving our mission forward. Fromvisionary leadersto dedicatedadministratorsand brilliantfaculty members the Institute is united by a shared passion for scientific exploration and innovation.

What Your Key Responsibilities Will Be

• Maintain expand passage cryopreserve and recover human iPSC lines using sterile technique.
• Prepare reagents and support multistage differentiation of iPSCs into pancreatic progenitors endocrine progenitors and beta cell or islet-like organoids.
• Monitor cell culture quality differentiation progress and maintain accurate experimental records.
• Assist with banking quality control and characterization of genetically engineered iPSC lines.
• Perform DNA RNA and protein extraction and assist with molecular and cellular assays including PCR RT-qPCR immunoblotting ELISA immunofluorescence flow cytometry and microscopy.
• Support analysis of beta cell differentiation and function through marker expression viability assays insulin/C-peptide measurements and glucose-stimulated insulin secretion assays.
• Maintain accurate sample records inventories and experimental data.
• Assist with preparation and transplantation of iPSC-derived beta cell clusters or islet-like organoids under approved animal protocols.
• Assist with animal handling monitoring dosing blood glucose measurements and sample collection following appropriate training.
• Support tissue collection processing and analysis while maintaining required animal and experimental records.
• Prepare media buffers solutions and reagents; maintain laboratory supplies equipment and inventory.
• Assist with ordering receiving labeling and storage of laboratory materials.
• Follow laboratory safety procedures and participate in meetings data discussions and troubleshooting activities.
• Performs other related duties as assigned by management.

Supervisory Responsibilities:

• This job has no supervisory responsibilities.

What we Require

• No professional experience required.
• Education: Bachelors degree in Biological Sciences or related scientific discipline required. A minimum of 2 years of experience in a scientific role is required for consideration of a Bachelors degree in a different discipline.
• Computer skills required: (Select all applicable): Accounting Software; Contract Management Systems; Development Software; Human Resource Systems; Inventory Software; Microsoft Office; Payroll Systems; Project Management Software)
• Other skills (preferred):
  • Prior experience or internships in a laboratory setting.
  • Prior experience with histology which may be gained from coursework and/or work experience.
  • Basic knowledge of laboratory procedures and safety practices.
  • Familiarity with specific laboratory techniques or equipment relevant to the Institutes operations.
  • Knowledge of relevant regulations and compliance standards.
  • Academic laboratory experience applying basic cell biology molecular biology biochemistry or tissue culture principles.
  • Experience maintaining organized laboratory records and following written protocols accurately.

What We Can Offer

The expected pay range for this position is $18.00 to $21.00 per hour.Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position the qualifications of the selected candidate departmental budget availability internal equity and external market pay for comparable jobs.

Benefits

Salk Institute offers competitive benefits including medical dental vision retirement paid time off tuition reimbursement patient advocacy services and transit/parking program.

Salk Values

The Salk Community both scientists and administrators worked together to define values that we believe support Salks pursuit of excellence. To be truly the best scientific institution requires not only incredible discoveries but a common understanding of how we should work together to enable those discoveries.

The acronym I CARE provides a simple way to remember each of the values and reminds each of us of the importance of what we do each day.

Equal Employment Opportunity Statement

The Salk Institute for Biological Studies is an Equal Opportunity Employer and is committed to providing equal access to opportunities for students employees applicants for employment and other visitors. Salk has also adopted and maintains a policy to encourage professional and respectful workplace behavior and prevent discriminatory and harassing conduct in our workplace.

Accordingly the Institute prohibits harassment and discrimination in employment on the basis of and considers all qualified applicants for employment without regard to actual or perceived race (race is inclusive of traits associated with race including but not limited to hair texture and protective hairstyles. Protective hairstyles include but not limited to such hairstyles as braids and twists) color religion religious creed (including religious dress and grooming practices) national origin ancestry citizenship physical or mental disability medical condition (including cancer and genetic characteristics) genetic information marital status age sex (including pregnancy childbirth breastfeeding or related medical conditions) reproductive health decision making gender gender identity gender expression sexual orientation veteran and/or military status (disabled veteran veteran of the Vietnam era other covered veteran status) political affiliation and any other status protected by state or federal law.

Discrimination is prohibited with any intersectionality of the above-mentioned characteristics including:

1. Any combination of characteristics.
2. A perception that the person has any of the characteristics or any combination of those characteristics.
3. A perception that the person is associated with a person who has or is perceived to have any of those characteristics or any combination of those characteristics.

Required Experience:

Junior IC