Next Generation Sequencing (NGS) Specialist I

Job SummaryThe Next Generation Sequencing (NGS) Core Facility at LJI is looking for a detail-oriented NGS Specialist I to conduct NGS workflows. The successful candidate will deliver high-quality data to internal and external researchers and perform duties in a reliable and reproducible manner. The NGS Specialist I is responsible for processing multiple sample types for the complete NGS workflow including cell handling RNA and DNA extraction library preparation and sequencing. Additional responsibilities include working with a core team of specialists in the operation and maintenance of equipment including multiple liquid handler platforms. The ideal candidate will possess excellent written and verbal communication skills be adaptable and have the ability to who complete a successful virtual screening will be asked to complete a skills-based assessment onsite as part of the interview process. Main Responsibilities Cell handling including washing and countingNucleic acid extractionLibrary preparationNext Generation SequencingMaintain detailed daily records in electronic notebooks Communicating regularly (via email and verbally) with users and customersTroubleshoot issues in all steps of the workflow as necessary; from sample receipt through data delivery (II level)Interpret library fragment shapes and sizes among different assays (cDNA RNA WGS PCR-Free WGS ATAC histone CUTnRUN Transcription factor CUTnRUN ChIP Input ChIP methylation ChIP Acetylation etc)Have basic bioinformatics skills or the willingness and ability to learnAble to download FASTQ files and basic command line and shell scriptingPlan experiments to optimize current protocols or to add capabilities (II-III)Program robotic tools to automate protocols Validate those automated protocols (II-III)Preferred SkillsNucleic acid extraction and purificationDNA and RNA library prep methods Quality control methods (BioAnalyzer Qubit TapeStation etc)Experience with labware handlingExperience with Illumina and 10X Genomics desirable Working knowledge of PCR assaysAbility to write and validate assay protocols and ability to advise users on assay development Be willing to foster and maintain collaborative relationships within the aware of and enthusiastic about incorporating future technology into current and future scientific projects and and maintenance of automated liquid handlers Minimum QualificationsSkilled pipetting techniqueBachelors degree in Biology or related field preferred0-2 years of experience in a biology laboratory (Tech I)