Highly qualified molecular biologist specialized in genomics for more than nine years with Team supervising and Lab management experience. Worked on population genetics and NGS assay validation research projects. Experie…
Highly qualified molecular biologist specialized in genomics for more than nine years with Team supervising and Lab management experience. Worked on population genetics and NGS assay validation research projects. Experienced in Next Generation Sequencing (NGS) on the Illumina Miseq, Ion Torrent, MGI, Nanopore sequencing platforms, Sanger Sequencing and DNA profiling. Having analytical and problem-solving skills, and technical and administrative and Lab management skills. Seeking a challenging technical / research /management position that will capitalize my skills, education, and experience.
Chip seq, nanopore seq, illumina seq, ddPCR, q pcr, cell culture,lab admin and management. Short and long read sequencing, 10x Genomics, single cell seq, tapestation, bioanalyzer, DNA/RNA extraction, sample servicing , training students etc
<p>Sample analysis services for researchers and non-academic users<br>
ChIP sequencing by Illumina<br>
Oxford Nanopore (MinION) sequencing<br>
ATAC sequencing by 10X Genomics<br>
Cell Culture of Suspension and Adherent cell lines (K562 & HCT116)<br>
Administrative support and Lab management<br>
ddPCR training and sample analysis for researchers and non-academic users</p>
<p>Working mainly on technical and application support, technical troubleshoots, and product promotions<br>
MGI- NGS sequencing Instruments and library prep kits<br>
MGI Automatic sample Handler for RNA extraction and analysis for Covid RT-PCR testing and Covid qPCR assays<br>
Cytometry Analysis</p>
<p>Worked mainly on technical and application support, technical troubleshoots and product promotions<br>
Thermo Fisher NGS assay applications library prep and technical troubleshoots for Ion Torrent Chef and GeneStudio S5 series platforms<br>
Sanger sequencing assays for single gene mutation/variant analysis using Seqstudio, ABI3500 and ABI3730<br>
TaqMan assays for gene expression and SNP detection using ABI7500 and Quantstudio 5, 7 Flux<br>
STR genotyping training and assay technical troubleshoots for HID and clinical applications<br>
Robotic liquid handling Instruments like ThermoFisher -KingFisher, Pathotrix Auto, Hamilton NGS star and Qiagen & Promega Instruments<br>
GenDX Chimerism qPCR-based assays<br>
All other Thermo and other collaborated company products<br>
Fast Track Diagnostics (FTD) qPCR based clinical assays for pathogen detection</p>
<p>Worked mainly on the assay validation, Tech transfer, and Quality Management of techniques to expand the Bangalore lab activities<br>
MLPA assays for Micro/Major deletion<br>
Sanger sequencing assays for single gene mutation/variant analysis<br>
Fragment analysis assays for mutation detection and confirmation<br>
NGS assay mutation detection and sanger confirmation<br>
Maternal Cell Contamination assays<br>
Validation report writing and SOP preparation as per CAP guidance<br>
Managing and supervising a team for project completion and reporting</p>
<p>Worked on different research projects like the ‘Validation of Cell-Free DNA (cfDNA) Assay for Liquid Biopsy samples’ and ‘Validation of 152 Somatic Cancer Gene Assay’<br>
In charge of the 152 Somatic Gene panel production samples as well<br>
Managing and maintaining the production samples received for 152 Somatic Gene panel assay<br>
Validated the different mutations and gene changes like amplification, deletion and translocation by using qPCR and Sanger sequencing methods<br>
Involved in the standardization of the 152 Somatic Gene panel for detecting the somatic mutations at the level of 5% to below 2% in NGS and copy number and translocation (fusion) changes<br>
Involved in the Inter/Intra operator/kit etc test for validating the assay<br>
Experienced in the various DNA library preparation methods for NGS sequencing<br>
Limit of Detection (LOD) studies of the custom-designed cancer panel on various cell line and Coriell DNA samples for their mutation detection level in the selected gene target sequences, different DNA and library quantization methods<br>
Involved in validating the assay for cfDNA extraction, recovery and mutation detection by ddPCR single gene and multiplex gene assays<br>
ddPCR assay confirmation of the mutation found in the NGS data and RNA extraction and cDNA conversion for qPCR assays<br>
Standardized an alternative method for FFPE Infinium QC Assay and qPCR-based assay for GC rich regions for confirming mutations<br>
Overall, Lab Maintenance and supervising the junior employees and lab Assistants</p>
<p>Worked on the research project named ‘Analysis of Degraded Human DNA by Autosomal Markers with Short Amplicons’<br>
Performed the DNA Extraction, Quantization, Genotyping and Sequencing of STR and SNP markers in the population samples and real samples for population genetic research studies and case reporting<br>
Polymorphic 16 mini/midi STR and 52 SNP loci were selected and analyzed in the collected eleven endogamous population samples of India<br>
Generated the polymorphic autosomal miniSTR & autosomal SNP panels for HID /population Genetic studies<br>
DNA database had been created for the selected Indian populations<br>
Worked on the analysis of the real case samples for DNA reporting<br>
Trained the Scientific Officers on DNA profiling techniques for human identification<br>
Participated in various national conferences and presented papers</p>
<p>Worked as a Lecturer in the Department of Behavioral Health and Allied Sciences to teach B.Sc, M.Sc. and PG Diploma of Forensic Biotechnology students<br>
Taught M.Sc., B.Sc. and PG diploma students for basic biological science subjects and DNA technologies<br>
Took Special Lectures and practical training classes for the Judges and police professionals for DNA profiling<br>
Participated and organized the conferences and workshops conducted in the university<br>
Participated in the curriculum and admission processes</p>
<p>Worked under the Lab Systems, Mumbai to assist in the data analysis and reports<br>
Assisted in the analysis of data collection, analysis and drafting reports</p>
<p>Worked on the Pilot project named ‘Blood mercury concentration in the fisherman populations’<br>
Detected blood mercury level in the fisherman population using UV spectrometry and Mercury Analyzer<br>
Prepared SOPs for various assays</p>
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