Looking for a full-time position, where I can demonstrate my technical and experimental skills and contribute to the development and better understanding of science for benefit.
Senior Research Scientist holds 20 years of experience. Over ten years of experience in cloning of various Biosimilar molecules, followed by the development and execution of cell based bioassays.
Expertise includes DNA extraction, cloning, expression, and transfection/electroporation using mammalian cell line to obtain stable clones. Agarose gel electrophoresis, DNA sequencing, ELISA, PCR, SDS-PAGE and Western blotting, Immunogenicity assay, Mammalian cell culture (Revival of cell lines, Passage, Maintenance and Preparation of Cell bank).
Cell based Bioassay: Development and standardization of cell based assays based on the mechanism of action for biosimilar molecules (monoclonal antibodies and proteins), ADCC, CDC
I am a goal-oriented with attention to details and can meet deadlines.
I can work independently as well as in a team and effectively supervise and mentor junior associates.
I look forward to learn and adapt new methods/technologies very comfortably.
Efficiently conduct independent or group laboratory research and accurately record data.
Ability to identify antibody protein sequences from Patents.
SAP: Purchase requisition, Service Purchase requisition, Material Reservation and Service entry and Generation of YEXP.
Protein Chemistry: Standard protein purification techniques (by using His-tag columns), SDS-PAGE and Western Blotting technique, Silver Staining, Coomassie staining. ELISA
Cell Culture: Cell culture (mammalian cell lines). Revival, passage and cell banking. Transfection and Electroporation to obtain stable clones.
<p>Conducts English lessons and day care.<br>
Helping design weekly lesson plans.</p>
<p>Molecular Biology - DNA extractions, cloning, DNA purification, Agarose gel electrophoresis, PCR, Transformations, DNA sequencing, Transfections<br>
Standard protein purification techniques (by using His-tag columns), SDS-PAGE and Western Blotting, Silver Staining, Coomassie staining<br>
Cell Culture - Cell culture techniques (mammalian cell lines). Revival, expansion, and cell banking.<br>
Development and standardization of cell-based bioassay for various biosimilar molecules.<br>
Efficiently conduct independent or group laboratory research and maintain electronic records accurately using eLN in a timely manner.<br>
Record and analyze data<br>
Ability to identify antibody / protein sequences from Patents<br>
Preparation of Reports for cross functional team.<br>
Results and conclusion presentation with project leader<br>
Proficiency with Microsoft Word, Excel, Power Point<br>
Ability to mentor junior colleagues<br>
SAP - Prepare Purchase requisition, Service Purchase requisition, Reservation, Service entries, YEXP proposal</p>
<p>Collection and performance of test<br>
Management of laboratory records<br>
Management of the appointment system</p>
<p>Creates lesson plans that are appropriate for the age and abilities of the students in the class<br>
Evaluates student performances in class and on test and provides feedback<br>
Assigns special projects to students<br>
Communicates students’ progress to parents, particularly regarding students who need tutoring or other extra assistance<br>
Ensures that computer equipment is functioning properly</p>
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