Ganiu Idris

<ul><li>Supervised the Medium and Reagent preparations</li><li>team</li><li>Supervised the Isolation of PBMCs and cryopreservation for immunological studies</li><li>Spearhead the cell culture department for all cell-based assays</li><li>Carried out Culture of PBMC cell isolates</li><li>Participated in Flow cytometry assays</li><li>Writing of Standard Operating Procedures for Immunological and serology assay</li><li>Conducted training on ELISA assay</li><li>Verification of assay data and transmission to analysis team</li><li>Reporting laboratory research experiment</li><li>Supervision of Postgraduates bench work relating to immunology and molecular process</li><li>Serving in company’s Quality Management System team as Equipment Officer</li></ul><p></p>

<p>Safety and Immunogenicity of Fractional Doses of Moderna, AstraZeneca &Janssen COVID-19 Vaccine Among Nigerian Adults (SIFCoVAN Clinical Trial Research Project, NIMR) </p><ul><li>Medium and Reagent preparations</li><li>Carried out the Isolation of PBMCs and cryopreservation </li><li>Carried out the Culture of PBMC cell isolates</li><li>Participated in Flow cytometry assays</li><li>Conducted Immunological training on ELISA assay</li><li>Verification of assay data and transmission to analysis team</li><li>Reporting laboratory research experiment</li><li>Writing assay report</li><li>Sample data entry, sorting, and primary analysis</li></ul><p>Making orders and requests for lab reagents and consumables.</p>

<p>Nigerian Institute of Medical Research (NIMR) </p><p>Cell culture lab, Center for Human Virology and Genomics</p><ul><li>Coordinated the collection of sewage contaminated water samples (SCW) from different sample sites in Lagos.</li><li> Conducted the Processing and concentration of collected SCW samples.</li><li>Frequently carried out the maintenance of mammalian cell culture.</li><li>Conducted the Inoculation of cultured cells with concentrated SCW samples (suspected virus inoculum).</li><li>Carried out the microscopic examination of infected cell lines for cytopathic effect (CPE).</li><li>Carried out Molecular analysis (CDNA, PCR).</li><li>Carried out gel electrophoresis.</li><li>Conducted the maintenance of the cell culture laboratory.</li><li>Making requests from the store.</li><li>Writing standard operating procedures and reports.</li></ul><p></p>

<p>VTM team lead COVID-19 Response team </p><ul><li>Conducted the preparation, packaging, and delivery of viral transport media (VTM) for NIMR’s COVID-19 sample collection and for commercial use across the nation.</li><li>Preparation of cell culture media and other reagents.</li><li>Participated in cytotoxicity assays.</li><li>Carried out SARS-CoV-2 isolation in mammalian cells.</li><li>Carried out TCID-50 and plaque assay.</li><li>Writing an experiment report for Senior research fellows.</li></ul><p></p>

<p>Standard operating Procedure development for Laboratory Assays<br>
Carried out PBMC isolation and cryopreservation<br>
Carried out COVID-19 antibody and cytokine Elisa assay<br>
Sorting and logging of received immunological samples data<br>
Primary Data sorting and analysis<br>
Transmission of primary dataset to Secondary data analysis team<br>
Making orders and requests for lab reagents and consumables</p>

<p>Standard operating Procedure development for Laboratory Assays<br>
Specialized in cell culturing and maintenance of cultured cell line for TCID assay, virus culture, plaque assay<br>
Carried out Cytotoxicity assay, drug efficacy test etc.<br>
Coordinated the Viral transport media production team of the Nigeria Institute of Medical Research COVID-19 response team<br>
Serves in capacity of assistant researcher in NIMR Enterovirus (Poliovirus surveillance research)<br>
Coordinated the collection of sewage contaminated water sample<br>
Processing and concentration of viral suspected sample<br>
Nucleic acid Extraction<br>
support in PCR assay<br>
Gel electrophoresis</p>