Research Technologist Zadeh Limited Tenure

Sets up and performs routine and established lab procedures in support of lab goals grant commitments i.e. assays cell/tissue culture animal studies etc. Monitors results reports unusual outcomes and suggests modifications. Collects enters and summarizes data. Assists in the maintenance of lab equipment and supplies. Performs basic statistical data analysis; prepares tables/charts/graphs and assists in organizing data. Presents at research laboratory meetings and contributes to writing of manuscripts. Works under supervision and may perform some assignments independently as discussed and approved by Principal Investigator.

Additional Information
We are seeking a highly motivated and detail-oriented Research Technologist to join our translational neuro-oncology research team. Our lab focuses on glioblastoma and peripheral nerve sheath tumors with a strong emphasis on tumor microenvironment biology spatial and single-cell transcriptomics hypoxia signaling and molecular biomarker discovery.

The successful candidate will support ongoing projects by performing established and emerging laboratory techniques including but not limited to:

Molecular biology assays (PCR Western blotting RNA/DNA extraction qPCR)
Immunohistochemistry and immunofluorescence staining on FFPE and frozen tissues
Human and murine cell culture (including primary tumor-derived lines)
Fluorescence and brightfield microscopy image acquisition and analysis
Mouse handling and tissue processing (training provided if needed)
Sample preparation for spatial transcriptomics single-cell analysis and next-generation sequencing
Data entry and assistance with manuscript and figure preparation
Organization of lab inventory reagents and general lab maintenance
The Research Technologist will present data in lab meetings assist with experimental planning and collaborate closely with senior lab members and trainees. The candidate will be expected to work independently on routine procedures and receive mentorship for more complex tasks. Opportunities for authorship on publications are available based on contributions.

Requires bachelors degree in biology chemistry other relevant science or an associates degree with 6 years of relevant laboratory experience. Must exhibit a solid understanding of lab techniques equipment and safety. Must have the ability to organize and carry out laboratory techniques independently and in a team setting. Experience with basic data analysis is required. This position requires demonstrated writing skills.

Preferred Qualifications

Bachelors degree in biology biomedical sciences or a related field is required (Masters degree or relevant research experience preferred)
Minimum of 2 year of hands-on wet lab experience (academic or industry); associate degree with extensive relevant lab experience will also be considered
Proficiency in standard molecular biology techniques and mammalian cell culture
Proficiency in Western blotting RNA/DNA extraction qPCR and basic data analysis (GraphPad ImageJ Excel/R)
Familiarity with gene silencing/knockout methods (CRISPR system shRNA siRNA)
Excellent organizational skills attention to detail and ability to work both independently and collaboratively
Strong communication and documentation skills
Experience with tissue sectioning digital pathology or prior exposure to spatial transcriptomics or single-cell workflows is an asset but not required. Training will be provided.
References

Three letters of recommendation will be required following an interview