Senior Scientist-Psychiatry Dr. Kaji
Job Location:
New York City, NY - USA
Monthly Salary:
Posted on:
Vacancies:
Job Summary
The laboratory studies human brain development and neuropsychiatric disease using stem cellderived organoids and primary fetal tissue. Current projects focus on:
- Defining how radial glia give rise to hippocampal and neocortical lineages and identifying signaling pathways (e.g. Wnt and beyond) that govern hippocampal subfield specification
- Building and characterizing human hippocampal organoid systems including efforts to engineer missing subfields (e.g. CA1/CA3/DG)
- Identifying conserved molecular and cellular programs across genetic and pharmacologic models of schizophrenia using large-scale single-cell and multiomic datasets
- Modeling neurodevelopmental contributions to schizophrenia through perturbation systems including chronic and acute NMDA receptor inhibition (e.g. ketamine exposure)
Developing scalable perturbation platforms (e.g. inducible CRISPRa/i systems) to systematically interrogate candidate regulatory pathways emerging from integrative analyses
optimization and execution of hiPSC culture reprogramming and differentiation workflows
Generation and maintenance of 3D brain organoids (neocortical and hippocampal) including protocol development for patterning and subfield specification
Implementation and standardization of signaling perturbation paradigms (e.g. Wnt modulation small molecules growth factors)
Development of inducible genetic perturbation systems (e.g. CRISPRa/i doxycycline-regulated constructs)
Processing of organoid and fetal tissue samples for downstream applications including:
single-nucleus RNA sequencing (snRNA-seq)
single-nucleus ATAC sequencing (snATAC-seq)
multiome assays
spatial transcriptomics
Organoid characterization using:
immunohistochemistry and confocal imaging
structural analysis of rosettes and laminar organization
Establishment and enforcement of standard operating procedures (SOPs) to ensure reproducibility and quality control across experiments
Evaluation and implementation of laboratory automation platforms to increase throughput and consistency
Supervision and training of research staff; coordination of day-to-day laboratory operations
Close collaboration with computational scientists to iteratively refine experimental design based on multiomic analyses
Qualifications
Educational and other Requirements for the position:
- Ph.D. in stem cell biology developmental biology neuroscience or a related field
- Significant experience working with human pluripotent stem cells (hESCs or hiPSCs)
- Demonstrated expertise in organoid systems or complex differentiation platforms
- Strong background in experimental design assay development and troubleshooting
- Excellent organizational and leadership skills with the ability to manage multiple parallel projects
Interest in building scalable reproducible research systems rather than executing isolated experiments
Experience Required:
- Extensive experience in iPSC culture reprogramming and lineage-specific differentiation
- Proven track record of developing or optimizing complex biological workflows
- Experience with genome engineering approaches (e.g. CRISPR/Cas9 inducible systems)
- Familiarity with single-cell or spatial genomics workflows (sample preparation level)
- Experience establishing SOPs and quality control pipelines in a research or core facility setting
- Prior experience supervising personnel or managing shared research resources is strongly preferred
- Experience with laboratory automation platforms is highly desirable
Required Experience:
Senior IC
About Company
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