Intern- Process Development

AskBio Inc. a wholly owned and independently operated subsidiary of Bayer AG is a fully integrated gene therapy company dedicated to developing life-saving medicines and changing lives. The company maintains a portfolio of clinical programs across a range of neuromuscular central nervous system cardiovascular and metabolic disease indications with a clinical-stage pipeline that includes investigational therapeutics for congestive heart failure limb-girdle muscular dystrophy multiple system atrophy Parkinsons disease and Pompe disease. AskBios gene therapy platform includes Pro10 an industry-leading proprietary cell line manufacturing process and an extensive array of capsids and promoters. With global headquarters in Research Triangle Park North Carolina and European headquarters in Edinburgh Scotland the company has generated hundreds of proprietary capsids and promoters several of which have entered pre-clinical and clinical testing.

Our vision: Pioneering science to create transformative molecular medicines.

Our mission: Lead innovative science and drive clinical outcomes to transform peoples lives.

Our principles:

• Advance innovative science by pushing boundaries.

• Bring transformative therapeutics to patients in need.

• Provide an environment for employees to reach their fullest potential.

Our values:

• Be a Pioneer. We are not afraid of the impossible and to innovate to make gene therapies accessible to those in need.

• Cultivate Collaboration. Strive to be the best teammate actively listen openly communicate and embrace diverse points of view.

• Embrace Responsibility. We are humbled by the enormity of our hold a relentless commitment to advance science and clinical outcomes for our patients families and caregivers.

• Raise the Bar. Continuously drive improvements and efficiencies. Seek and provide constructive feedback. Have a bias for learning and action.

• Act with Uncompromising Integrity. Be honest transparent and committed to doing whats right in every situation. Make clear commitments and follow through.

Position Summary

The Process Development intern will help utilize a high-throughput screening method to optimize upstream transfection conditions for rAAV production. The applicant will aseptically culture mammalian cells at small scale platforms such as shake flasks. The applicant will help screen transfection conditions to improve rAAV productivity.

The Intern Process Developments responsibilities will include aseptically culturing the mammalian cells in different cell culture platforms transfecting harvesting transfected cells and running characterization assays to fundamentally understand the transfection process. The candidate is expected to have prior experience with aseptic techniques general lab techniques such as pipetting and preparing solutions. Additionally the intern will be responsible for analyzing data and reporting results. The intern will be expected to give a formal presentation at the end of their internship tenure.

This intern will be based on site in RTP NC and will report to a Research Associate in Process Development.

Job Responsibilities

• Learn fundamentals of cell culture vector production transfection Dynamic Light Scattering (DLS) principles and other related subjects from training material

• Use aseptic techniques to culture HEK cells at small scale platforms (shake flask)

• Use good lab practices to prepare salt and buffer solutions through titrations

• Conduct viral vector production in small-scale cell culture models

• Learn & operate DLS and Flow Cytometer plate readers

• Attention to detail. Communicate findings and assist with data modelling

• Present findings and accomplishments in a formal presentation

Minimum Requirements

• Must be pursuing a Bachelors degree in engineering or science field (Chemical or Biological Engineering Biology Biomanufacturing Biotech Biochemistry major and/or cell culture experience preferred)

• Must be at least a sophomore in university at the time of your application

• Required aseptic technique and pipetting experience

• Proficient with MS Office Suite and ability to learn new software quickly

• Available to work full-time in person at RTP lab during summer months (May-August) at an AskBio office

Preferred Education Experience and Skills

• Prior internship work or volunteer/extracurricular experience preferred

• Knowledge of suspension mammalian cell culture benchtop bioreactor operation transfection (transient or stable) gene therapy viral vector production and cell flow cytometry

• Excellent verbal and written communication skills

• Strong sense of accountability organization skills record keeping skills and ability to prioritize multiple tasks (with strong attention to detail)

• Proactive and solution-driven mindset

AskBio Inc. (AskBio) is an Equal Opportunity Employer and does not discriminate against any employee or applicant for employment because of race color religion gender sexual orientation gender identity national origin age disability veteran status or any other protected status prohibited under Federal State or local laws. All employment decisions are based on valid jobrelated requirements. If you are a qualified individual with a disability or a disabled veteran and are unable or limited in your ability to use or access our website you may request a reasonable accommodation to express interest in a specific opening by calling us at or sending us an email at .

Agencies: Please do not contact any employee at AskBio about this requisition. Any resume submitted by a recruitment agency to any employee at AskBio through any medium will be deemed the sole property of AskBio unless the agency was engaged by AskBio Talent Acquisition team to recruit for that position. All agencies must have a prior executed service agreement with AskBio prior to any search engagement. If a candidate who was submitted outside of the AskBio agency process is hired by AskBio no fee or payment of any kind will be paid to the agency.