Research Assistant I NEUROLOGY KL
Research Assistant I NEUROLOGY KL
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Job Description
Mass General Brigham relies on a wide range of professionals including doctors nurses business people tech experts researchers and systems analysts to advance our mission. As a not-for-profit we support patient care research teaching and community service striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.
Job Summary
SummaryThe candidate will work independently under the supervision of Vikram Khurana MD PhD and a senior researcher in the Khurana lab (instructor/assistant professor). Depending on the specific project needs and candidate skill set the role will range from molecular biology focused bench work to development and optimization of assays for viability/toxicity in iPSC (induced pluripotent stem cells) derived neurons. These assays will include techniques such as flow cytometry longitudinal imaging of neurons plate-based live cell and calorimetric assays and western blotting. The candidate is expected to be diligent highly organized and collegial. Contact Dr. Daniel El Kodsi for inquiries:
Provides assistance to the Principal Investigator and Study Team in coordinating research activities that may include conducting a variety of routine experiments with established methodologies data collection and management and other research activities as assigned.
Candidates who are in the process of completing their bachelors degree have a grace period of six months from their hire date (up to 1 year if starting per diem)to provide degree equivalency verification.
Does this position require Patient Care
No
Essential Functions
- Maintenance passaging cataloguing and culturing of iPSC cells.
- Differentiation iPSCs to neurons and glia and aging of these cultures.
- Maintenance of Attune flow cytometry and ordering the related reagents
- Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope
- Maintenance of HEK293 cells generation of stable cell lines.
- Helping with molecular cloning; Gibson assembly gateway cloning mini/maxi preps
- The candidate will analyze data from western blot/ microscopy/ flow cytometry
- The candidate will have excellent lab note-keeping habits mostly digital (LabArchieves) and will generate Standard Operating Procedures (SOPs) for the lab
- The candidate will have excellent organizational skills labeling of experiments file management
- The candidate will report daily to the immediate supervisor and weekly to the Principal investigator.
- The candidate will interact with junior and senior member of the lab to troubleshoot experiments.
- The candidate will share lab duties with other technicians.
- Other assignments might be given depending on the project needs.
- Responsible for data collection management manipulation analysis and reporting.
- Study cohort management.
- Outreach communication with subjects conducting mailings follow-up on mailings and obtaining necessary documents.
- Provides assistance on work related to epidemiological studies and other population-based research.
Qualifications
Education
Bachelors Degree Science required
Experience
New grad with some relevant coursework 0-1 year preferred
Knowledge Skills and Abilities
- Good cell culture experience with human cells (HEK293 or any other immortalized line)
- iPSC stem cell culture experience is a plus but not required.
- Excellent cell culture practice/sterile techniques are necessary.
- Medium to high level of computer literacy and a basic understanding of statistical concepts are needed.
- Basic understanding of molecular techniques microscopy flow cytometry is desired.
- Understanding of basic molecular concepts: molecular cloning western blot RNA isolation qPCR western blot is desired.
- Excellent oral and written communication skills.
- High interpersonal skills are required for working in a highly diverse lab.
- Excellent organizational skills and ability to prioritize a variety of tasks.
- Excellent data management skills and attention to details.
- Good presentation skills are a plus.
- Careful attention to detail.
- Computer literacy including database tools.
- Ability to follow directions and exhibit professionalism.
Additional Job Details (if applicable)
Remote Type
Work Location
Scheduled Weekly Hours
Employee Type
Work Shift
Pay Range
$19.76 - $28.44/HourlyGrade
5EEO Statement:
Mass General Brigham Competency Framework
At Mass General Brigham our competency framework defines what effective leadership looks like by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance make hiring decisions identify development needs mobilize employees across our system and establish a strong talent pipeline.
Employment Type
Full-Time
