Senior Scientist, Cell Pharmacology

Senior Scientist Cell Pharmacology

Xilio Therapeutics is a clinical-stage biotechnology company discovering and developing tumor-activated
immuno-oncology (I-O) therapies with the goal of significantly improving outcomes for people living
with cancer without the systemic side effects of current I-O treatments. The company is using its
proprietary platform to advance a pipeline of novel tumor-activated clinical and preclinical I-O
molecules that are designed to optimize the therapeutic index by localizing anti-tumor activity within
the tumor microenvironment. Learn more by visiting and follow us on LinkedIn (Xilio
Therapeutics Inc.).

SUMMARY:

The Senior Scientist Cell Pharmacology is responsible for the development and execution of cell-based
in vitro and ex vivo assays to support our immune-oncology pipeline. This key role offers the opportunity
to directly contribute to Xilios cutting edge science by developing innovative assay systems and
generating decision-driving data that drive our programs forward. This role provides an exciting
opportunity to directly influence ongoing programs and contribute to the discovery of new drugs to
treat cancer patients. The pay range for this role is $126000-$147500.

Responsibilities:

Work as an integrated member of the research team to generate data to support ongoing drug
discovery efforts.
Serve as an expert in the development of functional cell-based assays to support discovery
programs and test Xilio molecule activation.
Develop and implement automation using liquid handling robotics to enhance throughput for
cell-based and cell-free in vitro assays.
Implement and execute cell-based experiments such as phenotypic assays and reporter gene
assays (RGA) which assess drug candidate activity and mechanism of action.
Develop and run binding assays via flow cytometry ELISA and MSD.
Culture primary human tumor and immune cells for ex vivo experiments.
Carry out enzymatic cleavage experiments with recombinant proteases.
Perform gel electrophoresis and western blot assays to assess recombinant proteins.
Research relevant literature to devise cellular functional experiments. Implement develop and
perform in vitro assays to characterize novel therapeutic proteins
Integrate technologies that improve or expand the ability to assay molecules by working with
teammates collaborators vendors and staying abreast of the literature.
Enter all data into laboratory notebooks and databases daily
Participation and presentation of data at group meetings.
Mentor junior scientists.
Represent cell pharmacology function at project team meetings.
Provide assay development expertise to support CMC non-clinical DMPK and Tox studies.

Skills Qualifications and Requirements:

BS/MS/PhD in the field of Biology or other related fields 7 years post-graduate experience with BS
5 years post-graduate experience with MS or 3 years experience with a PhD
Demonstrated strong background in IO / Immunology / T-Cell Engagers or a related field.
Experience with automation / liquid handlers for mid to high throughput assays strongly preferred.
Previous experience with Flow Cytometry ELISA and MSD-based readouts.
Skilled in cell/tissue culture techniques and cell-based assays
Experience with culture of primary human tumor and immune cells and Western blots
Technical experience in the development qualification and execution of assays for protease
characterization in human tissues
Goal oriented organized capable of working on multiple projects and able to execute to timelines
that align with company goals
Willing to learn new techniques and skills
Work well in a cross functional team environment
Good verbal and written communication skills
Primary tumor processing experience is preferred
Experience with measuring enzymatic activity is preferred

Equal Opportunity and Inclusion

Our human capital resources objectives include as applicable identifying recruiting retaining
incentivizing and developing our existing and additional employees. We are committed to equal
opportunity and inclusion across all aspects of our organization including in our recruitment
advancement and development practices. We conduct annual performance and development reviews
for each of our employees to discuss the individuals strengths and development opportunities career
development goals and performance goals. We also regularly survey employees to assess employee
engagement and addition each regular full-time employee is provided an allowance and
time to attend appropriate job-related trainings and other professional development courses seminars
meetings and similar sessions. If you are an individual with a disability who requires an accommodation
with the hiring process please reach out to