Biologist

Statement of Work Details

Provides technical experience needed to assist in studying the basic principles of plant and animal life

• Study basic principles of plant and animal life such as origin relationship development anatomy and function
• Identify classify and study structure behaviour ecology physiology nutrition culture and distribution of plant and animal species

Assists with performing basic laboratory experiments and procedures

• Collect and analyze biological data about relationships among and between organisms and their environment 4
• Monitor and observe experiments recording production and test data for evaluation by research personnel 5
• Analyze experimental data and interpret results to write reports and summaries of findings
• Cultivate maintain expand and freeze ES and iPS cell lines from mouse and human.
• Prepare ES and iPS cells for N-linked glycoprotein labelling and analysis by Mass Spectrometry.
• Prepare ES and iPS cells for flow analyses including the labelling of the cells with appropriate antibodies.
• Generate primary cultures of cells for the generation of new iPS cell lines.
• Characterize new iPS cell lines using appropriate techniques (morphology staining growth traits).
• Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based techniques.
• Perform primary cell cultures of mitotically inactivated feeder layers.
• Design and execute flow cytometry (FACS) preps proliferation assay and cell surface receptor analysis.
• Investigate state of pluripotency of ES and new iPS cell lines.
• Characterize nuclear proteins and cell surface proteins by western blot immunoprecipitation immunochemistry and PCR.
• Design primers as needed for cell characterization.
• Apply a wide range of conventional established approaches methods techniques and solutions to new situations; identify/recommend resolution of discrepancies; recommend/ determine what data to use record or report.

Prepares organizes and maintains various lab samples supplies and equipment

• Maintain laboratory equipment
• Analyze laboratory findings
• Maintain an accurate research tracking system and database for research activities; keep accurate records of daily experiments and results including research documentation.
• Prepare data results reports as well as Excel spreadsheets and PowerPoint slides for oral presentation and/or publication in journals; write and edit scientific papers.
• Attend monthly laboratory meetings; participate in lab/unit/section meetings by presenting ongoing research and findings; present paper/abstract/poster at scientific meeting(s)/conference(s).
• Operate and perform routine maintenance on laboratory instruments and equipment; ensure scientific supplies are kept stocked; obtain quotes and prepare purchase requests for supplies instruments and equipment and submit to primary investigator and/or lab manager for approval.

Learns new laboratory techniques and procedures

• Program and use computers to store process and analyze data 1
• Interpret research or operational data 2
• Record research or operational data 3

Other

• Cultivate maintain expand and freeze ES and iPS cell lines from mouse and human.- Prepare ES and iPS cells for N-linked glycoprotein labelling and analysis by Mass Spectrometry.
• Prepare ES and iPS cells for flow analyses including the labelling of the cells with appropriate antibodies.
• Generate primary cultures of cells for the generation of new iPS cell lines.
• Characterize new iPS cell lines using appropriate techniques (morphology staining growth traits).
• Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based techniques.
• Perform primary cell cultures of mitotically inactivated feeder layers.
• Design and execute flow cytometry (FACS) preps proliferation assay and cell surface receptor analysis.
• Investigate state of pluripotency of ES and new iPS cell lines.
• Characterize nuclear proteins and cell surface proteins by western blot immunoprecipitation immunochemistry and PCR.
• Design primers as needed for cell characterization.
• Apply a wide range of conventional established approaches methods techniques and solutions to new situations; identify/recommend resolution of discrepancies; recommend/ determine what data to use record or report.
• Maintain an accurate research tracking system and database for research activities; keep accurate records of daily experiments and results including research documentation. - Prepare data results reports as well as Excel spreadsheets and PowerPoint slides for oral presentation and/or publication in journals; write and edit scientific papers. - Attend monthly laboratory meetings; participate in lab/unit/section meetings by presenting ongoing research and findings; present paper/abstract/poster at scientific meeting(s)/conference(s).
• Operate and perform routine maintenance on laboratory instruments and equipment; ensure scientific supplies are kept stocked; obtain quotes and prepare purchase requests for supplies instruments and equipment and submit to primary investigator and/or lab manager for approval.