Biological Scientist

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Travel Required:

Clearance Required:

We are looking for a Biological Scientist to support advanced molecular cellular genomic and translational research programs for NIH. This position is responsible for designing executing and optimizing laboratory studies utilizing genome engineering stem cell biology high-throughput screening sequencing imaging and functional genomics technologies. This position is a full-time position located in Rockville MD.

What You Will Do:

This position is responsible for designing executing and optimizing laboratory studies utilizing genome engineering stem cell biology high-throughput screening sequencing imaging and functional genomics technologies.

• Design execute and optimize molecular biology experiments using gene expression analysis and functional genomics; design and generate genetic constructs through molecular cloning plasmid engineering CRISPR/Cas genome editing and viral vector preparation.

• Isolate quantify and assess DNA RNA and proteins using manual and automated workflows; prepare and process samples including library preparation and quality control for PCR qPCR NGS transcriptomics and single-cell sequencing to generate genomic and transcriptomic data. Coordinate downstream analyses with bioinformatics teams.

• Coordinate cell line quality control including mycoplasma testing karyotyping STR/DNA fingerprinting contamination monitoring and genetically modified cell line validation; manage cell banking activities including cryopreservation recovery authentication distribution shipment receipt and documentation.

• Perform and optimize mammalian cell culture experiments with primary cells stem cells immortalized cell lines and differentiated populations; differentiate stem cells into specialized cell types using established and novel protocols automated culture systems and high-throughput platforms.

• Perform protein characterization and cellular analyses using Western blotting immunocytochemistry and immunofluorescence staining; prepare and analyze tissue specimens using cryostat sectioning microscopy and histological techniques. Perform fluorescence and confocal imaging and quantitative image analysis.

• Develop validate and implement fluorescence- bioluminescence- and reporter-based high-throughput screening assays; perform cellular functional assays using flow cytometers Seahorse metabolic flux analyzers and multi-electrode arrays.

• Collect analyze and interpret experimental data; prepare technical reports manuscripts scientific publications and presentations and support grant applications. Participate in weekly laboratory meetings to present project updates findings and research progress.

• Maintain inventories of reagents antibodies plasmids cell lines consumables and experimental resources; operate calibrate troubleshoot and maintain laboratory instrumentation and analytical equipment including fluorescence and confocal imaging systems. Monitor laboratory operations and infrastructure and coordinate maintenance and instrument services.

• Prepare and maintain protocols SOPs electronic laboratory notebooks regulatory and technical documentation and quality records to ensure compliance and reproducibility; train laboratory personnel on equipment operation laboratory procedures and best practices.
• Provide multidisciplinary teams with expertise in molecular biology genome engineering stem cell biology assay development and translational research to advance program objectives and collaborative research initiatives.

What You Will Need:

• Masters degree in Biology Molecular Biology or a related life sciences field.

• Minimum of ONE (1) year of experience performing molecular biology techniques including cloning gene expression analysis and genome engineering.

• Background supporting cell culture stem cell biology and cellular assay development activities.

• Practical experience with genomic technologies including PCR qPCR NGS transcriptomics and sequencing workflows.

• Prior work analyzing experimental data and preparing technical reports and scientific documentation.

• Hands-on experience operating laboratory instrumentation and maintaining laboratory quality standards.

• Knowledge of laboratory safety procedures quality control practices and research documentation requirements.
• Ability to work effectively in a multidisciplinary research environment.
• Must be able to obtain and maintain a Federal or DoD public trust; candidates must receive approved adjudication prior to onboarding with Guidehouse. Candidates with an active public trust or suitability are preferred.

What Would Be Nice To Have:

• Prior work in a federal research environment.
• Strong scientific communication organization and collaboration skills.
  

What We Offer:

Guidehouse offers a comprehensive total rewards package that includes competitive compensation and a flexible benefits package that reflects our commitment to creating a diverse and supportive workplace.

Benefits include:

• Medical Rx Dental & Vision Insurance

• Personal and Family Sick Time & Company Paid Holidays

• Parental Leave

• 401(k) Retirement Plan

• Group Term Life and Travel Assistance

• Voluntary Life and AD&D Insurance

• Health Savings Account Health Care & Dependent Care Flexible Spending Accounts

• Transit and Parking Commuter Benefits

• Short-Term & Long-Term Disability

• Tuition Reimbursement Personal Development Certifications & Learning Opportunities

• Employee Referral Program

• Corporate Sponsored Events & Community Outreach

• annual membership

• Employee Assistance Program

• Supplemental Benefits via Corestream (Critical Care Hospital Indemnity Accident Insurance Legal Assistance and ID theft protection etc.)

• Position may be eligible for a discretionary variable incentive bonus

About Guidehouse

Guidehouse is an Equal Opportunity EmployerProtected Veterans Individuals with Disabilities or any other basis protected by law ordinance or regulation.

Guidehouse will consider for employment qualified applicants with criminal histories in a manner consistent with the requirements of applicable law or ordinance including the Fair Chance Ordinance of Los Angeles and San Francisco.

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