Research Scientist I Protein Sciences and Structural Biology

Embedded within the Broad Institute the Center for the Development of Therapeutics (CDoT) is a highly collaborative organization of professional drug discovery scientists. Our mission is to translate cutting edge biological insights originating from the Broad community into impactful drug discovery projects. We partner with faculty from MIT Harvard and the Harvard teaching hospitals together with industry partners in pharmaceutical biotechnology and venture capital firms to validate druggable targets and identify leads. We combine our deep expertise in drug discovery with the knowledge and unique capabilities of the Broad Institute to explore drug discovery projects in a wide range of disease areas (including oncology cardiovascular psychiatry infectious and rare diseases). We use systematic approaches in genomics biology high content imaging chemistry and machine learning coupled with the Broads culture of creativity risk taking and open sharing to successfully advance science and develop new treatments for patients.

We are seeking a passionate and highly motivated Research Scientist I to join CDoTs Protein Technologies team. The successful candidate will have a solid understanding of protein construct design expression purification and quality assessment. Experience in structural biology is advantageous.

Principle Duties and Responsibilities:

• Developing and executing strategies to clone and express recombinant proteins in a variety of expression systems
• Purifying tagged or native proteins using standard purification (FPLC systems and column chromatography) and analytical techniques (SDS-PAGE Western blot light scattering and protein LC/MS)
• With training produce high quality protein crystal structures and co-crystal structures carrying out the process from crystallization screening through to structure refinement
• Initiating and completing routine protein construct design and production in an independent and efficient manner
• Demonstrating the ability to interpret experimental outcomes and proposing follow-up studies
• Troubleshooting and solving scientific problems related to protein production independently
• Operating and maintaining lab equipment independently including protein purification systems like an AKTA pure
• Investigating new technologies related to protein expression and purification
• Documenting work and effectively presenting results in cross-functional project teams

• Ph.D in Biophysics Biochemistry Molecular Biology Structural Biology or related life science field with 0-2 years of research experience in an industrial or academic setting
• Expertise in recombinant protein purification using chromatography (affinity ion exchange and size exclusion)
• Significant experience with cloning and expressing proteins using different vectors tags and various cell culture systems (mammalian insect bacterial yeast)
• Significant experience with protein characterization (analytical size exclusion chromatography mass spectrometry light scattering western blot and biophysical binding studies)
• Experience in protein crystallography and crystallographic data processing model refinement and structural analysis is a plus
• Experience with biophysical characterization of proteins (ITC DSC SPR NMR etc.) is a plus
• A proven record of successful interaction with project team members external customers or vendors