Downstream Purification Scientist
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Job Location:
Boston, NH - USA
Hourly Salary:
$ 46 - 47
Posted on:
3 hours ago
Vacancies:
1 Vacancy
Job Summary
Downstream Purification Scientist needs 2 years of hands-on purification experience in an academic or industry setting
Downstream Purification Scientist requires:
- Hands-on experience in Cesium chloride (CsCl) density gradient ultracentrifugation ion-exchange chromatography affinity purification and tangential flow filtration (TFF) with a strong understanding of purification principles and data analysis.
- Masters degree in biology biochemistry chemical engineering biotechnology or related field.
- AAV or other viral vector CsCl density gradient and column purification.
- Experience with AKTA systems and Unicorn software.
Downstream Purification Scientist duties:
- Execute purification processes including CsCl density gradient ultracentrifugation affinity chromatography (e.g. AVB CaptureSelect) ion-exchange chromatography (AEX CEX) and other polishing steps.
- Operate AKTA systems (Avant Pure or Pilot) for small- to large-scale purification runs. Perform TFF operations for buffer exchange and concentration of intermediate or final products.
- Support experimental design data collection and documentation following established procedures and GMP/GLP best practices.
- Assist in troubleshooting and optimization of purification processes.
- Analyze in-process and purified samples using relevant analytical methods (e.g. UV SDS-PAGE ddPCR ELISA or HPLC).
- Collaborate cross-functionally with upstream analytical and manufacturing teams.
- Maintain lab equipment manage buffers and column inventory and ensure compliance with safety and documentation standards.
