Research Assistant II Laboratory of Dr. Christian Metallo

The Research Assistant II will support the laboratory of Dr. Christian Metallo. They will focus on research related to stem cell and organoid-based stem cell models of the brain that will be used to study the metabolism of human glial cells in various disease conditions. This research on iPSC cultures and organoids will be conducted under the supervision of Dr. Metallo and a post-doctoral fellow and will comprise the main duties of this addition to differentiating and maintaining iPSC cultures other duties may include media preparation plating passaging and freezing cells for 2D tissue culture preparation of samples and loading samples for mass spectrometry analysis basic mass spectrometry equipment maintenance (under supervision) molecular biology techniques with RNA and DNA Western blots general lab maintenance and collaboration with other lab members.

Who We Are

The Salk Institute is an internationally renowned research institution that valuesallmembers of our scientific community. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces perspectives across discipline professional acumen and unique life experiences fostering innovation and a sense of belonging. Together we strive to improve the wellbeing of humanity through groundbreaking research.

Founded by Jonas Salk developer of the first safe and effective polio vaccine the Institute is an independent nonprofit research organization and architectural landmark: small by choice intimate by nature and fearless in the face of any challenge. Salks vibrant community has many talented individuals from varied backgrounds each playing a crucial role in driving our mission forward. Fromvisionary leadersto dedicatedadministratorsand brilliantfaculty members the Institute is united by a shared passion for scientific exploration and innovation.

What Your Key Responsibilities Will Be

• Perform standard mammalian cell culture including regular maintenance of iPSC lines.
• Execute differentiation experiments to generate glial and neuronal organoids.
• Plate passage thaw and freeze cells to maintain 2D tissue culture lines bank cells and generate cells for experiments.
• Maintain tissue culture lab space for cultivation and differentiation of pluripotent stem cell models of neurological disease.
• Perform basic molecular biology procedures for gene and protein analysis.
• Execute metabolite and protein extractions from cells organoids and biospecimens.
• Analyze samples and perform basic maintenance of gas chromatography liquid chromatography and mass spectrometry instruments.
• Perform duties to support experiments including mentoring new staff and students.
• Assist with operational support for laboratory including space and equipment.
• Modify procedures for specific research and contribute to new techniques and protocols.
• Ensure data quality and integrity.
• Make and document detailed observations and exercise sound judgement in task execution.
• Attend meetings to discuss future research direction.
• Performs other related duties as assigned by management.

What we Require

• Minimum 2 years experience or equivalent combination of education and experience required.
• Bachelors degree in Biological Sciences or related scientific discipline required. A minimum of 4 years of experience in a scientific role is required for consideration of a Bachelors degree in a different discipline.
• Computer skills required: Microsoft Office. Statistical analysis graphing and presentation of data.

What We Can Offer

The expected pay range for this position is $21.00 to $25.00 hourly.Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position the qualifications of the selected candidate departmental budget availability internal equity geographic location and external market pay for comparable jobs.

Benefits

Salk Institute offers competitivebenefits including medical dental vision retirement paid time off tuition reimbursement patient advocacy services and transit/parking program.

Salk Values

The Salk Community both scientists and administrators worked together to define values that we believe support Salks pursuit of excellence. To be truly the best scientific institution requires not only incredible discoveries but a common understanding of how we should work together to enable those discoveries.

The acronymI CAREprovides a simple way to remember each of the values and reminds each of us of the importance of what we do each day.

Equal Employment Opportunity Statement

The Salk Institute for Biological Studies is an Equal Opportunity Employer and is committed to providing equal access to opportunities for students employees applicants for employment and other visitors. Salk has also adopted and maintains a policy to encourage professional and respectful workplace behavior and prevent discriminatory and harassing conduct in our workplace.

Accordingly the Institute prohibits harassment and discrimination in employment on the basis of and considers all qualified applicants for employment without regard to actual or perceived race (race is inclusive of traits associated with race including but not limited to hair texture and protective hairstyles. Protective hairstyles include but not limited to such hairstyles as braids and twists) color religion religious creed (including religious dress and grooming practices) national origin ancestry citizenship physical or mental disability medical condition (including cancer and genetic characteristics) genetic information marital status age sex (including pregnancy childbirth breastfeeding or related medical conditions) reproductive health decision making gender gender identity gender expression sexual orientation veteran and/or military status (disabled veteran veteran of the Vietnam era other covered veteran status) political affiliation and any other status protected by state or federal law.

Discrimination is prohibited with any intersectionality of the above-mentioned characteristics including:

1. Any combination of characteristics.
2. A perception that the person has any of the characteristics or any combination of those characteristics.
3. A perception that the person is associated with a person who has or is perceived to have any of those characteristics or any combination of those characteristics.

Required Experience:

Junior IC