Core Rsch Facil Specialist I NextGen DNA Sequencing

Core Rsch Facil Specialist I

Technical Functions

• Perform basic assays that are part of quality control and sample preparation processes for NGS including but not limited to: quantification (QUBIT FLUOstar Omega BMG Nanodrop) sizing (TapeStation 4150 and TapeStation 4200) size selection and cleanup (SAGE-ELF electrophoretic agarose gels AMPure magnetic bead purification) and DNA fragmentation (Covaris S220 and Covaris E220).
• Construct and perform quality assessment of Illumina PacBio and Nanopore libraries.
• Plan validate set up and execute Illumina MiSeq i100 NovaSeq X Plus PacBio Revio and/or Nanopore MinION and PromethION sequencing runs.
• Maintain Illumina MiSeq i100 NovaSeq X Plus PacBio Revio and Nanopore MinION/PromethION instruments and troubleshoot causes of failed runs.
• Coordinate repair services for Illumina MiSeq i100 NovaSeq X Plus PacBio Revio and the Nanopore MinION and PromethION instruments and support equipment (Covaris S220 and Covaris E220).
• Perform verification validation and troubleshooting of work processes as needed for Illumina PacBio and Nanopore platforms.
• Document all work by using established lab tracking sheets (LTS) and digital documentation tools (FileShare).
• Develop adapt test troubleshoot and implement new protocols for NGS library construction and other NGS workflows using novel procedures as they become available in the literature or from vendors.
• Communicate with supervisor and other core personnel about experimental results in a timely and professional manner.
• Work with supervisor to define deliverables and timelines for project completion.
• Consult and communicate with internal and external clients in a clear and professional manner regarding experimental planning services and troubleshooting.
• Perform NGS sample intake functions using ICBR-LIMS such as receiving and logging samples or requests for services.
• Contribute to the preparation updating and organization of Standard Operating Procedures (SOPs).

General Lab Operation and Maintenance Functions

• Assist Scientific Director with administrative duties necessary for a fee-for-service facility operation i.e. fee/charge entry.
• Participate in hiring staff and managing staff performance to ensure staff are compliant with UF policies and procedures.
• Contribute to the maintenance of a complete inventory of reagents consumables and equipment used for NGS sequencing.
• Assist in training and assessment of new staff.
• Lead efforts at evaluating new laboratory methods for the implementation of new services in the NGS core.
• Revision of sample sheets tracking and disposal of samples in MyBiotech.
• Freeze and fridge maintenance and documentation in MyBiotech.
• Contribute to the maintenance of a complete inventory of reagents consumables and equipment used for NGS sequencing.
• Perform maintenance operations for the TapeStation (i.e. periodic restart of software).
• Help in laboratory organization cleaning and general maintenance.
• Receive store and document orders arriving at the lab.
• Help send off outgoing parcels when requested

Educational Functions

• Keep abreast of new developments in the NGS field through the literature and scientific meetings.
• Help in the training of other personnel in the NGS Core group.
• Participate in cross-training activities in the NGS core and ICBR as necessary or as requested by the lab director.
• Other duties as assigned.

$51000 to $53000

BA/BS and 3 years of related experience; or an equivalent combination of education and experience.
Core or technology-specific licensure and/or certifications where applicable. Additionally yearly certificate and compliance training if required.

Genomic DNA purification quantification and manipulation PCR NGS DNA library construction and quality assessment/evaluation.
Use of robotics for high throughput processing of molecular biology samples.
Hands-on experience with Illumina PacBio and/or ONT NGS instrumentation and technology.
Basic laboratory citizenship including organization of equipment and supply inventory
Instrument run coordination and maintenance.
Preparation of common buffers and solutions.
Computer literacy in Windows/UNIX systems Microsoft office package.
Standard Operating Procedures (SOPs) iLAB and laboratory information management system (LIMS).

In order to be considered you must upload your cover letter and resume.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.