Protein Sciences Research Scientist

Job Description

General Summary:

We are seeking a motivated Scientist to join our Protein Science this role you will purify and characterize therapeutic proteins and research reagents at milligram- to gram-scale in support of early- and late-stage discovery programs. The ideal candidate will bring strong expertise in protein purification protein analytics and protein-protein interaction characterization and will help advance platform capabilities through innovative approaches in analytical characterization and biologics developability assessment.

Key Duties and Responsibilities:

• Operate AKTA chromatography systems using UNICORN software to purify antibodies Fc fusion proteins and His-tagged protein reagents for in vitro and in vivo applications.
• Design and execute fit-for-purpose purification strategies using Protein A immobilized metal affinity chromatography (IMAC) ion exchange chromatography (IEX) hydrophobic interaction chromatography (HIC) and preparative size exclusion chromatography (SEC).
• Perform analytical characterization using UV-Vis spectroscopy analytical SEC on HPLC/UPLC systems with Empower software endotoxin testing SDS-PAGE and western blotting.
• Develop implement and improve analytical workflows to support the developability assessment of biologics.
• Collaborate with cross-functional teams and external partners to support mass spectrometry analyses.
• Develop and optimize bio-layer interferometry (BLI) assays to evaluate protein-protein interactions and determine binding kinetics including KD kon and koff.
• Present purification analytical and developability data to the Protein Science team project teams and the broader research organization.
• Maintain accurate timely and well-organized records in electronic lab notebooks and protein inventory/database systems.

Knowledge and Skills:

• Demonstrated expertise operating AKTA systems and UNICORN software for protein purification.
• Strong hands-on experience with protein capture and polishing methods using resins such as MabSelect SuRe MabSelect PrismA Capto L Capto DEAE Capto Q and Capto S.
• Proficiency with Waters HPLC/UPLC systems and Empower software including SEC HIC IEX and reversed-phase methods.
• Experience performing SDS-PAGE and developing or troubleshooting western blot methods.
• Experience evaluating protein stability and manufacturability using biophysical methods such as DSF DLS AC-SINS cross-interaction chromatography (CIC) or standup monolayer chromatography (SMAC).
• Experience supporting protein therapeutic candidates from early discovery through advancement toward development in highly collaborative fast-paced and results-driven teams.
• Experience using BLI or SPR to perform kinetic or quantitative binding assays.
• Familiarity with cIEF for charged variant analysis and mass spectrometry for protein identification and post-translational modification (PTM) analysis.

Education and Experience:

• PhD in Cell Biology Biochemistry Biophysics Chemical Engineering Bioengineering or a related field with 2 years of relevant industry experience; or
• Masters degree in one of the above fields with 3 years of relevant industry experience; or
• Bachelors degree in one of the above fields with 6 years of relevant industry experience.

Pay Range:

Disclosure Statement:

Flex Designation:

Flex Eligibility Status:

In this On-Site designated role you will work five days per week on-site with ad hoc flexibility.

Note: The Flex status for this position is subject to Vertexs Policy on Flex @ Vertex Program and may be changed at any time.

#LI-Onsite

Company Information

Vertex is a global biotechnology company that invests in scientific innovation.

Vertex is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a persons race color sex gender identity or expression age religion national origin ancestry ethnicity disability veteran status genetic information sexual orientation marital status or any characteristic protected under applicable law. Vertex is an E-Verify Employer in the United States. Vertex will make reasonable accommodations for qualified individuals with known disabilities in accordance with applicable law.

Any applicant requiring an accommodation in connection with the hiring process and/or to perform the essential functions of the position for which the applicant has applied should make a request to the recruiter or hiring manager or contact Talent Acquisition at