Research Assistant I NEUROLOGY krl
Boston, NH - USA
Job Summary
Mass General Brigham relies on a wide range of professionals including doctors nurses business people tech experts researchers and systems analysts to advance our mission. As a not-for-profit we support patient care research teaching and community service striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.
Job Summary
SummaryThe candidate will work independently under the supervision of Joanna Korecka PhD and a senior researcher in the Korecka lab (postdoctoral fellow). The role will range from molecular biology-focused bench work mouse tissue processing to development and optimization of assays for viability/toxicity in iPSC (induced pluripotent stem cells) derived neurons. These assays will include techniques such as flow cytometry longitudinal imaging of neurons plate-based live cell and calorimetric assays and western blotting. The candidate is expected to be diligent highly organized and collegial.
Position provides assistance to the Principal Investigator and Study Team in coordinating research activities that may include conducting a variety of routine experiments with established methodologies data collection and management and other research activities as assigned.
Does this position require Patient Care
No
Essential Functions
-Maintenance passaging cataloguing and culturing of iPSC cells.
-Differentiation iPSCs to neurons and glia and aging of these cultures.
-Maintenance of Attune flow cytometry and ordering the related reagents.
-Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope.
-Maintenance of HEK293 cells generation of stable cell lines.
-Helping with molecular cloning; Gibson assembly gateway cloning mini/maxi preps.
-Analysis of data from western blot/ microscopy/ flow cytometry.
- Mouse handling and pre and post-op care.
- Mouse tissue processing immunohistochemistry and confocal imaging.
- Live cell calcium and MEA (multielectrode array) computational data analysis.
-The candidate will have excellent lab note-keeping habits mostly digital(LabArchieves) and will generate Standard Operating Procedures (SOPs) for the lab.
-The candidate will have excellent organizational skills labeling of experiments file management.
-The candidate will report daily to the immediate supervisor and weekly to the Principal investigator.
-The candidate will interact with junior and senior members of the lab to troubleshoot experiments.
-The candidate will share lab duties with other technicians.
-Responsible for data collection management manipulation analysis and reporting.
-Study cohort management.
-Outreach communication with subjects conducting mailings follow-up on mailings and obtaining necessary documents.
-Provides assistance on work related to epidemiological studies and other population-based research.
-Performs other duties as assigned.
-Complies with all policies and standards.
Qualifications
Education
Bachelors Degree Science required
Can this role accept experience in lieu of a degree
Yes
Experience
New grad with some relevant coursework 0-1 year preferred
Knowledge Skills and Abilities
-Excellent oral and written communication skills.
-Strong interpersonal skills are required for working in a highly diverse lab.
-Excellent organizational skills and ability to prioritize a variety of tasks.
-Excellent data management skills and attention to detail.
-Good presentation skills are a plus.
-Computer literacy including database tools.
-Ability to follow directions and exhibit professionalism.
Additional Job Details (if applicable)
Remote Type
Work Location
Scheduled Weekly Hours
Employee Type
Work Shift
Pay Range
$20.16 - $29.01/HourlyGrade
5EEO Statement:
Mass General Brigham Competency Framework
At Mass General Brigham our competency framework defines what effective leadership looks like by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance make hiring decisions identify development needs mobilize employees across our system and establish a strong talent pipeline.
Required Experience:
Junior IC
About Company
Patients at Mass General have access to a vast network of physicians, nearly all of whom are Harvard Medical School faculty and many of whom are leaders within their fields.