Research Associate I
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Job Location:
San Diego, CA - USA
Monthly Salary:
Not Disclosed
Posted on:
2 hours ago
Vacancies:
1 Vacancy
Job Summary
Position Details:
Our client a world-leading Pharmaceutical Company in San Diego CA is currently looking for an Research Associate I to join their expanding team.
Job Title: Research Associate I / Cell Culture
Duration: 12 months contract extendable up to 48 months
Location: San Diego CA
Note:
The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.
You may participate in the company group medical insurance plan
Job Description:
Our client a world-leading Pharmaceutical Company in San Diego CA is currently looking for an Research Associate I to join their expanding team.
Job Title: Research Associate I / Cell Culture
Duration: 12 months contract extendable up to 48 months
Location: San Diego CA
Note:
The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.
You may participate in the company group medical insurance plan
Job Description:
Research Associate I
Position Summary
The Structural Biology and Protein Sciences group is seeking a highly motivated contractor to support recombinant protein production efforts primarily utilizing insect cell expression systems.
The successful candidate will be responsible for maintaining eukaryotic cell cultures generating and managing high-titer baculovirus stocks and performing multi-liter scale protein expression and harvest workflows.
Additional responsibilities include executing plate-based expression and purification profiling to enable rapid screening of construct and condition variants.
This role requires strong organizational skills attention to detail and the ability to operate efficiently in a fast-paced research environment.
The Structural Biology and Protein Sciences group is seeking a highly motivated contractor to support recombinant protein production efforts primarily utilizing insect cell expression systems.
The successful candidate will be responsible for maintaining eukaryotic cell cultures generating and managing high-titer baculovirus stocks and performing multi-liter scale protein expression and harvest workflows.
Additional responsibilities include executing plate-based expression and purification profiling to enable rapid screening of construct and condition variants.
This role requires strong organizational skills attention to detail and the ability to operate efficiently in a fast-paced research environment.
Key Responsibilities
Maintain healthy eukaryotic cell cultures including insect (Sf9 Sf21 High Five) and/or mammalian expression lines.
Generate amplify and quality-control baculovirus stocks including centralized storage and record management.
Execute multi-liter scale recombinant protein expression experiments including infection culture monitoring and cell harvest.
Perform small-scale plate-based expression and purification profiling to assess expression levels solubility and purification behavior.
Maintain accurate and timely records of experimental data cell line usage and virus stock inventories.
Operate and maintain basic laboratory instrumentation and contribute to general lab support activities as needed.
Collaborate closely with team members to optimize expression workflows and troubleshoot technical issues.
Maintain healthy eukaryotic cell cultures including insect (Sf9 Sf21 High Five) and/or mammalian expression lines.
Generate amplify and quality-control baculovirus stocks including centralized storage and record management.
Execute multi-liter scale recombinant protein expression experiments including infection culture monitoring and cell harvest.
Perform small-scale plate-based expression and purification profiling to assess expression levels solubility and purification behavior.
Maintain accurate and timely records of experimental data cell line usage and virus stock inventories.
Operate and maintain basic laboratory instrumentation and contribute to general lab support activities as needed.
Collaborate closely with team members to optimize expression workflows and troubleshoot technical issues.
Minimum Qualifications
BS degree in Molecular Biology Cell Biology Biochemistry or a related scientific discipline.
Minimum of 3 years of hands-on laboratory experience including work with insect cell culture (e.g. Sf9 Sf21 Hi5) and/or mammalian cell culture systems.
Demonstrated experience in aseptic technique and routine maintenance of suspension cultures.
BS degree in Molecular Biology Cell Biology Biochemistry or a related scientific discipline.
Minimum of 3 years of hands-on laboratory experience including work with insect cell culture (e.g. Sf9 Sf21 Hi5) and/or mammalian cell culture systems.
Demonstrated experience in aseptic technique and routine maintenance of suspension cultures.
Highly Desirable Qualifications
Experience with the Bac-to-Bac baculovirus expression system including donor plasmid preparation bacmid generation and virus amplification.
Familiarity with plate-based expression and purification approaches (e.g. 2496 well plates and rapid IMAC and other affinity-based screens).
Experience conducting multi-liter expression campaigns and associated downstream sample handling.
Strong analytical organizational and documentation skills.
Ability to work independently and in cross-functional teams.
Key Skills
- Laboratory Experience
- ELISA
- Immunoassays
- Mammalian Cell Culture
- Biochemistry
- Cell Biology
- Research Experience
- Cell Culture
- Molecular Biology
- Microscopy
- Research Laboratory Experience
- Western Blot
