This position is a key member of an immunology research team focused on investigating the biology and function of invariant natural killer T (iNKT) cells and leveraging their unique properties to develop novel immunotherapies. The role supports research efforts within the Gumperz lab and contributes to collaborative projects examining EpsteinBarr virus (EBV) pathogenesis and the development of immuneresistant cells for pancreatic islet transplantation. The successful candidate will participate in the design and execution of rigorous laboratory experiments contribute to data analysis and interpretation and support daytoday research activities. This position requires strong organizational communication and interpersonal skills a solid understanding of experimental design principles and enthusiasm for handson research and mentoring undergraduate trainees in a collaborative laboratory environment.
This position requires work to be completed onsite at a designated campus work location.
This position has been identified as a position of trust with access to vulnerable selected candidate will be required to pass an initial caregiver check to be eligible for employment under the Wisconsin Caregiver Law and every four years. Applicants for this position will be considered for the following titles Researcher I and Researcher II. The title is determined by the experience and qualifications of the finalist.
This vacancy is being announced simultaneously with job posting JR; please note that only one vacancy exists. Having two position vacancy listings allows the School of Medicine and Public Health to consider candidates with a variety of experience and qualifications.
Isolate and culture primary mammalian cells and other cell lines to support laboratory experiments and collaborative efforts; generate preparations of antigens; generate stocks of purified and/or labeled monoclonal antibodies recombinant fusion proteins and other in-house laboratory reagents.
Troubleshoot and solve problems related to research techniques and protocols used in the lab.
Carry out duties related to maintenance of mice for experimental use including management/oversight of ordering breeding and experimental colonies.
Supervise undergraduate student hourly workers and mentor undergraduates performing independent research projects in the lab.
Conduct independent laboratory research under the guidance of the PI.
Carry out duties related to laboratory management (e.g. oversight and ordering of supplies maintenance of equipment and laboratory stocks coordination of scheduling related to use of equipment and lab space training others on use of equipment as needed).
Assist in developing grant applications and proposals to secure research funding
The mission of the Department of Medical Microbiology & Immunology is to provide comprehensive scientific instruction to students conduct novel and meaningful research and offer consultation and service in microbiology and immunology as they relate to human health and disease.
The starting salary for this position is $47000; but is negotiable based on experiences and qualifications.
Employees in this position can expect to receive benefits such as generous vacation holidays and sick leave; competitive insurances and savings accounts; retirement benefits. For more information refer to the campus benefits webpage.
Researcher I:
At least 6 years hands-on laboratory research experience in immunology or a related biological science discipline
Experience isolating culturing and analyzing primary human immune cells (T cells and/or NK cells required; experience with monocytes macrophages or dendritic cells a plus)
Proficiency performing common immunoassays (e.g. ELISA ELISpot cytotoxicity lymphocyte proliferation)
Experience working with murine models including basic in vivo procedures and support of mouse colony maintenance (breeding genotyping husbandry)
Experience analyzing flow cytometry data using FlowJo or similar software
Researcher II:
At least 6 years handson laboratory research experience in immunology or a related biological science with the ability to independently execute experimental workflows
Experience isolating culturing and analyzing primary human immune cells including T cells and/or NK cells (experience with monocytes macrophages or dendritic cells beneficial)
Proficiency in performing a range of immunoassays such as ELISA ELISpot cytotoxicity assays and lymphocyte proliferation assays with the ability to troubleshoot and optimize assays as needed
Experience working with murine models including performing routine in vivo procedures and independently supporting mouse colony management (breeding genotyping and husbandry)
Experience conducting and interpreting flow cytometry experiments including data analysis using FlowJo or similar software
Researcher I Preferred Qualifications:
Experience isolating and culturing primary human tumor cells or tumorinfiltrating lymphocytes (TILs) including work with tumor spheroid/organoid cultures
Experience supporting in vitro assays to evaluate antitumor immune responses and familiarity with xenograft mouse models involving human tumor or immune cells
Experience with histology fluorescence microscopy and/or livecell imaging systems (e.g. IncuCyte)
Experience performing molecular biology techniques such as realtime PCR (qPCR) and transcriptomic/genomic analyses (e.g. RNAseq ATACseq ChIPseq)
Experience with protein analysis techniques including immunoprecipitation and Western blotting
Researcher II Preferred Qualifications:
Experience designing optimizing or troubleshooting assays involving primary human immune cells or related cellular models
Experience isolating and culturing primary human tumor cells or tumorinfiltrating lymphocytes (TILs) including work with spheroid or organoid cultures
Experience conducting in vitro assays to evaluate antitumor immune responses
Experience working with xenograft mouse models involving human tumor tissue or immune cells
Experience performing molecular biology techniques such as realtime PCR (qPCR) and transcriptomic/genomic methods (e.g. RNAseq ATACseq ChIPseq)
Experience with protein analysis techniques including immunoprecipitation and Western blotting
Demonstrated ability to independently analyze data troubleshoot technical issues and contribute to experimental planning
Strong organizational skills and experience coordinating research activities within a team environment
MS required majoring in Biological Sciences or equivalent.
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To apply for this position select either I am a current employee or I am not a current employee under Apply Now. You will then be prompted to upload your application materials.
Important: The application has only one attachment field. Upload the following documents in that field either as a single combined file or as multiple files in the same upload area.
Cover letter required
Resume
Your cover letter should address how your training and experience aligns with the required and preferred qualifications listed above. Application reviewers will rely on these written materials to determine which applicants move forward in the process. References will be requested from final candidates. All applicants will be notified once the search concludes and a candidate is selected
University sponsorship is not available for this position including transfers of sponsorship and TN selected applicant will be responsible for ensuring their continuous eligibility to work in the United States (i.e. a citizen or national of the United States a lawful permanent resident a foreign national authorized to work in the United States without the need of an employer sponsorship) on or before the effective date of appointment. This position is an ongoing position that will require continuous work you are selected for this position you must provide proof of work authorization and eligibility to work.
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