Protein Science Principal Research Associate
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Job Location:
Seattle, OR - USA
Monthly Salary:
$ 88000 - 132000
Posted on:
8 days ago
Vacancies:
1 Vacancy
Job Summary
Job Description
Protein Science Principal Research Associate (Protein Engineering Protein Production)
General/Position Summary
The Protein Sciences Principal Research Associate provides laboratory support in advancement of Protein Engineering pipeline with conducting cloning workflows and plasmid preparation. They work closely with protein engineers to provide support with yeast transformation yeast cultures and characterization by flow cytometry. They provide support for mammalian expressions and high-throughput purification and biophysical characterization of proteins as needed.
Key Duties & Responsibilities
- Execute and troubleshoot molecular cloning workflows spanning plasmid assembly to plasmid preparation at mini- and midi- scales
- Perform small-scale yeast transformations and culturing
- Support characterization of yeast displayed proteins by flow cytometry
- Support large-scale yeast transformations with DNA libraries
- Support lab operations and equipment purchasing if needed
- Perform high-throughput purification of proteins and protein-analytics using UV-Vis Spectroscopy analytical SEC (UPLC-HPLC through Empower software) endotoxin tests SDS-PAGE and western blotting
- Manage protein inventory in the protein database and Protein Science freezers
- Provide protein dilutions and protein aliquots to immunology group and pharmacology group as needed
- Deliver verbal and presentation updates on cloning expression purification and analytics to the Protein Science Team.
- Ensure that a complete and timely record of experiments constructs plasmid preps is maintained in both the protein database and electronic lab notebooks
Required Education Level
- Masters Degree (or equivalent degree) in cellular biology biochemistry biophysics or bio/chemical engineering and 3 years of relevant industry experience or
- Bachelors Degree (or equivalent degree) in cellular biology biochemistry biophysics or bio/chemical engineering and 6 years of relevant industry experience
Required Knowledge/Skills
- Experience using electronic lab notebooks cloning software such as snap gene
- Experience in working in a highly collaborative laboratory which is timeline-driven and result orientated team is a must
- Experience in mammalian cell culture wok to transiently express and purify recombinant proteins is a must
- Expertise in molecular biology techniques such as PCR DNA cloning is a must
Pay Range:
$88000 - $132000Disclosure Statement:
The range provided is based on what we believe is a reasonable estimate for the base salary pay range for this job at the time of posting. This role is eligible for an annual bonus and annual equity awards. Some roles may also be eligible for overtime pay in accordance with federal and state requirements. Actual base salary pay will be based on a number of factors including skills competencies experience and other job-related factors permitted by law.
At Vertex our Total Rewards offerings also include inclusive market-leading benefits to meet our employees wherever they are in their career financial family and wellbeing journey while providing flexibility and resources to support their growth and aspirations. From medical dental and vision benefits to generous paid time off (including a week-long company shutdown in the Summer and the Winter) educational assistance programs including student loan repayment a generous commuting subsidy matching charitable donations 401(k) and so much more.
Flex Designation:
On-Site DesignatedFlex Eligibility Status:
In this On-Site designated role you will work five days per week on-site with ad hoc flexibility.
Note: The Flex status for this position is subject to Vertexs Policy on Flex @ Vertex Program and may be changed at any time.
#LI-Onsite
Company Information
Vertex is a global biotechnology company that invests in scientific innovation.
Vertex is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a persons race color sex gender identity or expression age religion national origin ancestry ethnicity disability veteran status genetic information sexual orientation marital status or any characteristic protected under applicable law. Vertex is an E-Verify Employer in the United States. Vertex will make reasonable accommodations for qualified individuals with known disabilities in accordance with applicable law.
Any applicant requiring an accommodation in connection with the hiring process and/or to perform the essential functions of the position for which the applicant has applied should make a request to the recruiter or hiring manager or contact Talent Acquisition at
Required Experience:
Staff IC
Key Skills
- Laboratory Experience
- ELISA
- Immunoassays
- Mammalian Cell Culture
- Biochemistry
- Cell Biology
- Research Experience
- Cell Culture
- Molecular Biology
- Microscopy
- Research Laboratory Experience
- Western Blot
About Company
Vertex Pharmaceuticals invests in scientific innovation to create transformative medicines for people with serious diseases.
