DescriptionAbouttheWorkUnit:
The Karbstein Lab in the Department of Biochemistry at Vanderbilt University is seeking an enthusiastic molecular biology/biochemistry research assistant to aid in the labs studies of ribosome assembly quality control and addition to providing a better understanding about the cellular mechanisms underlying ribosome integrity this basic science work is highly relevant to cancer biology the biology of aging. The lab relies heavily on biochemistry and molecular biology as well as yeast genetics. For examples of the type of research that will be undertaken see: Blomqvist et al 2023 PLoS Genetics; Yang et al 2023 Mol Cell; and Parker et al 2024 PLoS Biology; Yang&Karbstein BioRxiv 2024.
The lab is a mid-sized lab seeking a research associate who will spend their time performing research with some time spent maintaining lab infrastructure. The new research associate will be directly mentored and instructed by Dr. Karbstein the Principle Investigator and plan conduct interpret and present research.The laboratory is a supportive fast-paced environment for driven candidates who enjoy a collaborative atmosphere.It is expected that the new research associate will produce enough data for a first author publication within 1-2 years of joining the lab.
The lab strives to be a diverse inclusive and equitable working environment; prioritizes personal growth and wellness; and is committed to work-life balance. Overall this is an excellent opportunity for an experienced researcher who enjoys the independence of an academic lab.
KeyFunctionsandExpectedPerformances:
Research and Development:
- Purifies ribosomes from various organisms for mass spectrometry analysis
- Experience in RNA biochemistry is an asset
- Uses protein gels Western blotting and mass spectrometry to analyze ribosome assembly factors and ribosomal proteins
- Uses Northern blotting to analyze ribosome assembly in cells
- Uses agarose gels to analyze PCR screens and PCR reactions
- Generates yeast and worm strains and human cell lines
- Uses quantitative growth measurements to monitor genetic interactions
- Designs and clones proteins and RNAs for yeast worm or human in vivo work and in vitro assays
- Analyzes RNAseq data
- Analyzes experiments independently and prepares them for presentation to the PI
SupervisoryRelationships:
EducationandCertifications:
- A Bachelors degree in cellular biology molecular biology biochemistry or a related field from an accredited institution of higher education and a minimum of four years of experience is required.
Experience andSkills:
- Ability to perform all basic calculations related to buffer composition (Molarity dilution factor etc.) is necessary.
- Experience with protein and/or RNA biochemistry is strongly preferred.
- Computational experience is preferred.
- Being comfortable in learning new assays is necessary.
Required Experience:
Junior IC
DescriptionAbouttheWorkUnit:The Karbstein Lab in the Department of Biochemistry at Vanderbilt University is seeking an enthusiastic molecular biology/biochemistry research assistant to aid in the labs studies of ribosome assembly quality control and addition to providing a better understanding abou...
DescriptionAbouttheWorkUnit:
The Karbstein Lab in the Department of Biochemistry at Vanderbilt University is seeking an enthusiastic molecular biology/biochemistry research assistant to aid in the labs studies of ribosome assembly quality control and addition to providing a better understanding about the cellular mechanisms underlying ribosome integrity this basic science work is highly relevant to cancer biology the biology of aging. The lab relies heavily on biochemistry and molecular biology as well as yeast genetics. For examples of the type of research that will be undertaken see: Blomqvist et al 2023 PLoS Genetics; Yang et al 2023 Mol Cell; and Parker et al 2024 PLoS Biology; Yang&Karbstein BioRxiv 2024.
The lab is a mid-sized lab seeking a research associate who will spend their time performing research with some time spent maintaining lab infrastructure. The new research associate will be directly mentored and instructed by Dr. Karbstein the Principle Investigator and plan conduct interpret and present research.The laboratory is a supportive fast-paced environment for driven candidates who enjoy a collaborative atmosphere.It is expected that the new research associate will produce enough data for a first author publication within 1-2 years of joining the lab.
The lab strives to be a diverse inclusive and equitable working environment; prioritizes personal growth and wellness; and is committed to work-life balance. Overall this is an excellent opportunity for an experienced researcher who enjoys the independence of an academic lab.
KeyFunctionsandExpectedPerformances:
Research and Development:
- Purifies ribosomes from various organisms for mass spectrometry analysis
- Experience in RNA biochemistry is an asset
- Uses protein gels Western blotting and mass spectrometry to analyze ribosome assembly factors and ribosomal proteins
- Uses Northern blotting to analyze ribosome assembly in cells
- Uses agarose gels to analyze PCR screens and PCR reactions
- Generates yeast and worm strains and human cell lines
- Uses quantitative growth measurements to monitor genetic interactions
- Designs and clones proteins and RNAs for yeast worm or human in vivo work and in vitro assays
- Analyzes RNAseq data
- Analyzes experiments independently and prepares them for presentation to the PI
SupervisoryRelationships:
EducationandCertifications:
- A Bachelors degree in cellular biology molecular biology biochemistry or a related field from an accredited institution of higher education and a minimum of four years of experience is required.
Experience andSkills:
- Ability to perform all basic calculations related to buffer composition (Molarity dilution factor etc.) is necessary.
- Experience with protein and/or RNA biochemistry is strongly preferred.
- Computational experience is preferred.
- Being comfortable in learning new assays is necessary.
Required Experience:
Junior IC
View more
View less