DescriptionFUNCTION OF JOB
High level biomedical research involving cutting edge and traditional biomolecular analyses of human and nonhuman primate cells tissues and fluids including tissue collection and processing tissue fractionation biomolecular purification and characterization histochemical and immunohistochemical analyses multimodal imaging and image analysis cardiovascular and musculoskeletal assessments cellular purification and analysis assessments of gene expression and special procedures. Under general supervision plan develop modify and implement previously developed and original experimental procedures and as necessary help develop specialized analytical procedures and report final analyses in a research laboratory as needed. Excellent communication and writing and analytical skills required.
CHARACTERISTIC WORK
- Confer with principal investigator and the research team in planning development and implementation of research projects; develop and maintain control procedures for test standardization and QC as needed. Write and maintain Standard Operating Procedures (SOPs).
- Carry out and develop experimental techniques for specimen and tissue analyses utilized in complex laboratory procedures (see specifics below).
- Review scientific journals abstracts and other research literature to obtain or verify additional information used in the design and development of original procedures or techniques related to the research/clinical projects.
- Maintain detailed records as necessary. Maintain and organize data and tissue resources for analysis. Analyze data and prepare reports on the results of experiments; discuss with principal investigator the interpretation of the results and prepare scientific manuscripts for publication.
- Perform other related duties incidental to the work described herein.
SPECIFIC DUTIES
- Extraction purification and characterization of DNA RNA and proteins from tissues and cultured cells. Quantitative analysis of mRNA / miRNA species by a variety of methods quantitative and qualitative analysis of small molecules and proteins by RIA ELISA and other methodologies.
- Special tissue preparation and processing for localization and quantitation of protein and nucleic acid molecules of interest including cryosectioning paraffin embedding and sectioning immunohistochemistry in situ hybridization in situ RTPCR as well as other technically demanding methods.
- Digital image collection and processing data compilation maintenance of laboratory supply inventory and radioisotope records.
- Occasional animal training handling imaging and necropsy as necessary including careful dissection preparation storage and subsequent maintenance of tissues obtained during surgeries and necropsies.Cataloging and oversight of tissue storage.
EDUCATION/EXPERIENCE
Bachelors degree in the biological and/or physical sciences with two years of related clinical/research experience; or an equivalent combination of education and experience. Masters degree in biology or related physical science preferred.
SKILLS/QUALIFICATIONS
- Ability to listen and communicate effectively
- Basic computer skills
- Knowledge of medical terminology to interact with medical staff regarding laboratory procedures and results
- Knowledge of medical equipment and instruments
- Knowledge of common safety hazards and universal precautions to establish a safe work environment
WORK ENVIRONMENT
- Research laboratory environment
- Frequent exposure to diseases medicinal preparations and other conditions common in a lab environment
- Exposure to glass and other breakable objects
- Exposure to animals
- Must wear protective equipment (gloves gowns/lab coats eye protection face protection)
Required Experience:
IC