Scientist II

Responsibilities:

• Excellent employment opportunity for a Scientist II in the Ridgefield, CT area.
• Design, plan and execute experiments in an independent manner leveraging scientific and technical expertise to achieve defined objectives.
• Independently initiates and carries out the process for assay development, optimization and validation as required.
• Culture cell lines and process primary tissues from different species.
• Utilize multi-color flow cytometry and mass cytometry (CyTOF) techniques to develop novel assays for target engagement and/or pharmacodynamics biomarkers.
• Work with lab supervisor to identify and optimize new and innovative assays associated with cytometry-based biomarker discovery.
• Utilize molecular techniques to aid in the understanding of drug candidate mode of action.
• Routine maintenance of laboratory equipment.
• Ordering of laboratory consumables to ensure continuous supplies.
• Design, execute, interpret and complete projects in a timely manner.
• Work well in a highly matrixed environment and support multiple projects at the same time.
• Works independently with the capability to troubleshoot experimental procedures.
• Provide training and guidance to laboratory personnel.
• Prepare technical reports, publications and oral presentations.
  

Experience:

• Bachelor's Degree from an accredited institution with three-plus (3+) years of experience in related Scientific discipline or Master's Degree from an accredited institution in related Scientific discipline or equivalent experience in related field.
• Mammalian cell culture expertise.
• Cell culture experience with both cell lines and primary immune cells (necessary).
• Practical experience with multi-color/ multi-parametric cytometry-based approaches, including cell preparation, staining and acquisition (highly desirable).
• Practical experience with general molecular biology techniques such as qPCR and nucleic acid extraction from tissues and cells is a plus.
• Proficiency with computers and familiarity with data analysis software (GraphPad, FlowJo, etc.) is a plus.
• Required Skills: MAMMALIAN CELL CULTURE, QPCR, CELL CULTURE, ASSAY DEVELOPMENT, DATA ANALYSIS
• Additional Skills: MOLECULAR BIOLOGY, OPTIMIZATION, STAINING, FLOW CYTOMETRY, LABORATORY, LABORATORY EQUIPMENT, MAINTENANCE, ORDERING, TRAINING