Scientist, RNA Chemistry

Dyne Therapeutics is focused on delivering functional improvement for people living with genetically driven neuromuscular diseases. We are developing therapeutics that target muscle and the central nervous system (CNS) to address the root cause of disease. The company is advancing clinical programs for Duchenne muscular dystrophy (DMD) and myotonic dystrophy type 1 (DM1) as well as a preclinical programs for facioscapulohumeral muscular dystrophy (FSHD) and Pompe disease. At Dyne we are on a mission to deliver functional improvement for individuals families and communities. Learn more at and follow us on X LinkedInand Facebook.

Role Summary:

The Scientist RNA Chemistry contributes to the development of Dynes oligonucleotide chemistry platform and supports the discovery of novel nucleic acid therapeutics. As a member of the Platform team this role independently designs and executes the synthesis purification and characterization of oligonucleotides and their conjugates. The Scientist applies established methodologies troubleshoots routine technical challenges and leverages expertise in modified RNA synthesis to produce high-quality oligonucleotides using state-of-the-art laboratory technologies.

This position is based in Waltham MA

Primary Responsibilities Include:

• Independentlyperform synthesis of modified RNA oligonucleotides(ASO siRNAs)incorporating diverse sugar and backbone chemistries at scales suitable for both in vitroand invivo studies.
• Troubleshoot andoptimizesynthesis methods to produce high quality oligonucleotides of various lengths.
• Interpretation of analytical data (HPLC LCMS)to enableimprovedpurity and yields of synthesized oligonucleotides.
• Carry outpurification (anion exchange reverse phase)analysis(IP/RP IEX SEC)characterization(LCMS UV melting temperature) of oligonucleotidesand their conjugates.
• Register synthesizedoligonucleotidesin thedatabase (sequencelot andQC)and precisely report experiments in the ELN.
• Contribute tothe design of oligonucleotides against various targetsand rationally performthehit-to-lead optimization process.
• Generate intermediates to prepare antibody-oligonucleotide conjugates.
• Cross-functional collaborationto define deliverables and timelines.
• Multi-task across several projects and manage time effectively to achieve results.
• Perform lab maintenance activities in collaboration with peer scientists and lab operations.
• Collect present and discuss data at internal team meetings.

Education and Skills Requirements:

• PhD in Chemistry or a related scientific disciplinewith 2 years of experience or MSc with a minimum of 8 years of relevant industry experience in biotechnology or life sciences. Postdoctoral experience is a plus
• Hands-on experience operating oligonucleotide synthesis and purification platforms includingMerMade K&A and AKTAOligopilot.
• Experience with oligonucleotide conjugation including antibody conjugation is a plus.
• Familiarity with biochemical and analytical assays used to assess stability properties and mechanism of action of modified RNA oligonucleotides.
• Independently executes experimental work within defined research projects applying sound scientificmethodologyto address increasingly complex problems.
• Interprets and communicates experimental data to support program advancement and decision-making.
• Demonstrates strong cross-functional collaboration skills working effectively with internal partners to advance shared scientificobjectives.

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The pay range reflects the base pay range Dyne reasonably expects to pay for this role at the time of posting. Individual compensation depends on factors such as education experience job-related knowledge and demonstrated skills.

The statements contained herein reflect general details as necessary to describe the principles functions for this job the level of knowledge and skill typically required and the scope of responsibility but should not be considered an all-inclusive listing of work requirements. Individuals may perform other duties as assigned including work in other functional areas to cover absences or relief to equalize peak work periods or otherwise balance workload.

Dyne Therapeutics is an equal opportunity employer and will not discriminate against any employee or applicant on the basis of age color disability gender national origin race religion sexual orientation veteran status or any classification protected by federal state or local law.