Scientist – Molecular Cloning & Automation

We are seeking a skilled Scientist Molecular Cloning & Automation to lead plasmid design DNA assembly and scalable workflow development. You will apply advanced cloning strategies and build automated solutions to support high-throughput construct generation ensuring quality efficiency and on-time delivery across multiple programs.

Job Responsibilities

• Design and execute end-to-end plasmid workflows translating protein or guide RNA sequences into verified production-ready constructs
• Select and apply appropriate cloning strategies (e.g. Golden Gate Gibson Assembly restriction-ligation) based on construct complexity
• Develop implement and scale automated workflows for high-throughput plasmid production including:
  • Liquid handling platforms (Hamilton STAR Opentrons Beckman Biomek)
  • Automated colony picking systems (e.g. QPix)
  • High-throughput miniprep processes
• Establish and manage tiered QC pipelines (colony PCR restriction digest Sanger and/or long-read sequencing) while maintaining accurate construct documentation
• Oversee CRO and vendor relationships for outsourced cloning and gene synthesis including construct submission timeline management and QC review
• Partner cross-functionally to deliver constructs on time managing multiple concurrent requests with clear proactive communication

Qualifications :

Required Qualifications:

• B.S. with 5 years M.S. with 3 years or Ph.D. with 2 years of relevant industry experience in Molecular Biology Biochemistry Bioengineering or a related field
• Expert proficiency in Golden Gate Gibson Assembly and restriction-ligation cloning with the ability to select optimal strategies
• Strong knowledge of plasmid biology including origins of replication selection markers promoter design codon optimization and management of recombination-prone sequences
• Hands-on PCR experience including polymerase selection (e.g. Q5 Phusion) primer design and troubleshooting challenging amplicons.
• Experience managing CRO/vendors for gene synthesis or outsourced cloning
• Proficiency with Benchling or similar ELN for construct tracking and QC documentation
• Familiarity with sequence design and visualization tools (e.g. SnapGene Geneious)
• Proven ability to manage multiple simultaneous cloning projects and deliver high-quality constructs on timeline
• Strong communication skills with a collaborative cross-functional mindset

Preferred Qualifications:

• Experience programming validating and troubleshooting liquid handling platforms (e.g. Hamilton Beckman Tecan Opentrons)
• Familiarity with automated colony picking and high-throughput plasmid prep workflows
• Experience with pooled oligo cloning including library design bias mitigation and NGS-based QC analysis
• Background in lentiviral or pooled library cloning workflows
• Experience with long-read sequencing technologies (PacBio or Nanopore)
• Exposure to gene therapy applications (AAV LVV mRNA CAR-T) and downstream construct use
• Experience supporting IVT template design or mRNA production workflows
• Authorization to work in the U.S. without sponsorship

Additional Information :

What to Expect in the Hiring Process: 

• 10-15 Minute Phone Interview with Region Recruiter
• 45-60 Minute Virtual Interview with Manager and/or Group Leader
• 30 Minute In-Person Onsite Meeting with the Team

Additional Details: 

The position is Full Time Monday-Friday 8am-5pm. Candidates currently living within a commutable distance of Boston MA are encouraged to apply.

Excellent full-time benefits include:

• Comprehensive medical coverage dental and vision options
• Life and disability insurance
• 401(k) with company match
• Paid vacation and holidays
• Hourly rate is between $45.00-$48.00 depending on education and experience 

Eurofins USA PSS Insourcing Solutions is a Disabled and Veteran Equal Employment Opportunity employer.

Remote Work :

No

Employment Type :

Full-time