Scientist III Cell Biology & Functional Genomics

Responsibilities:

• Excellent employment opportunity for a Scientist III Cell Biology & Functional Genomics in the Ridgefield CT area.
• The successful candidate will support discovery research programs within Cardiovascular-Renal-Metabolic Diseases through hands-on operation of laboratory automation platforms mammalian cell culture cell-based assays and functional genomics studies.
• The candidate will execute and monitor established automated cell culture and assay workflows and support automated iPSC-derived kidney organoid processes and next-generation sequencing (NGS)-related work with platform- and workflow-specific training provided as needed.
• The role requires reliable execution of automation and NGS workflows. Success requires exceptional attention to detail careful sample and reagent tracking precise adherence to established protocols and independent troubleshooting of routine experimental or platform issues.
• Design execute and analyze cell-based experiments supporting target validation and discovery research including routine mammalian cell culture assay execution image-based analysis and iPSC-derived cell workflows.
• Operate and monitor established laboratory automation workflows for cell-based and molecular assays troubleshoot routine issues and escalate complex problems appropriately.
• Perform tissue cell and nuclei dissociation and execute NGS workflows for bulk RNA-seq single-cell RNA-seq single-nucleus RNA-seq and related applications including library preparation quality control quantification normalization sequencing setup and sequencer operation.
• Perform routine molecular biology techniques including DNA/RNA extraction PCR and qPCR nucleic acid and protein quantification Western blotting and basic sample preparation; analyze and interpret results identify technical issues and communicate findings and recommended follow-up clearly.
• Maintain accurate sample reagent consumable experimental metadata and electronic laboratory notebook records while complying with SOPs safety requirements company policies and data integrity standards.

• Collaborate across cell biology automation genomics and bioinformatics groups.

  Experience:

• Masters degree with 3 years of relevant experience; or Bachelors degree with 7 years of relevant experience; or Equivalent combination of education and experience in cell biology molecular biology genomics or a related scientific discipline.
• Strong hands-on experience with mammalian cell culture cell biology and cell-based assay execution.
  Hands-on NGS workflow experience including tissue cell and/or nuclei dissociation sequencing library preparation quality control and quantification for bulk RNA-seq single-cell RNA-seq single-nucleus RNA-seq or related applications.
• Some hands-on experience with laboratory automation automated instrumentation or standardized high-throughput workflows with the ability and interest to develop further expertise in operating and troubleshooting automated cell-based and molecular assays; automation programming is not required.
• Exceptional attention to detail and demonstrated ability to accurately execute lengthy multi-step protocols while maintaining sample integrity data quality and complete documentation.
• Ability to independently troubleshoot routine experimental or operational issues communicate clearly and work effectively in a collaborative research environment.
  

Desired Skills

• Experience running laboratory automation platforms for cell-based or molecular assays.
• Experience with sequencing run setup and sequencer operation. Instrument-specific operation can be trained when required.
• Experience with human induced pluripotent stem cell culture differentiation characterization or iPSC-derived cell assays.
• Experience with kidney organoid culture systems.