Scientist II Viral Vector
South San Francisco, CA - USA
Job Summary
We are seeking a highly motivated Viral Vector Research Associate/Scientist with 510 years of laboratory research experience to support our Viral Vector Core and gene therapy research programs. The ideal candidate will have hands-on experience in viral vector production mammalian cell culture molecular biology and CRISPR genome editing technologies. This role involves the production and characterization of lentiviral and AAV vectors supporting next-generation viral vector engineering maintaining viral inventories and collaborating with cross-functional research teams to advance innovative gene therapy platforms.
- Produce purify and characterize lentiviral libraries and Adeno-associated virus (AAV) vectors to support research and preclinical studies.
- Perform viral vector analytics using molecular biology techniques including PCR qPCR ddPCR/dPCR Western blot and related assays.
- Maintain and manage viral vector inventory sample tracking and documentation using electronic laboratory notebooks (ELN) and digital informatics systems.
- Support the engineering optimization and development of next-generation viral vectors and emerging viral-based therapeutic modalities.
- Perform mammalian cell culture transient plasmid transfection cloning and other molecular biology techniques.
- Participate in CRISPR/Cas9 genome editing experiments for both in vitro and in vivo applications.
- Operate laboratory instrumentation including flow cytometers and support data collection analysis and interpretation.
- Maintain accurate laboratory documentation experimental records and inventory following established laboratory procedures.
- Collaborate with multidisciplinary research teams to support gene therapy virology and genome engineering projects.
- Analyze experimental data troubleshoot protocols and contribute to continuous process improvements.
- MS or PhD in Cell Biology Molecular Biology Virology Biochemistry Bioengineering Genetics or a related scientific discipline.
- 510 years of laboratory research experience in molecular biology virology or gene therapy.
- Hands-on experience with lentiviral vector production and/or AAV production.
- Strong experience with mammalian cell culture and transient plasmid transfection.
- Experience with molecular biology techniques including:
- PCR
- qPCR
- ddPCR / dPCR
- Western Blot
- PCR
- Experience with CRISPR/Cas9 genome editing technologies.
- Strong organizational analytical troubleshooting and communication skills.
- Experience with viral vector engineering and next-generation viral modalities.
- Experience operating flow cytometers.
- Familiarity with electronic laboratory notebooks (ELN) and digital sample management systems.
- Experience with data analysis computational programming and bioinformatics tools.
- Previous experience supporting gene therapy viral vector core facilities or translational research programs.
Required Skills:
Viral Vector Lenti Virus AVV