Scientist I (Gene Therapy Cell Cloning)

Our client a world leader in life sciences and biotechnology is looking for a Scientist I based out of San Diego CA.

Job Duration: Long Term Contract (Possibility Of Extension)

Pay Rate : $46.43/hr on W2

Company Benefits: Medical Dental Vision Paid Sick leave 401K

The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques including plasmid cloning in vitro validation and the production of in vitro-transcribed mRNA. To verify successful expression the scientist will utilize assays such as Western blot qRT-PCR and luciferase reporter assays.

Job Responsibilities:

Design and clone expression vectors and IVT donor plasmids to support research initiatives.

Execute DNA and mRNA transfections in mouse and human cell lines.

Assess nucleic acid expression through molecular methods like qPCR reporter assays and Western blotting.

Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

Required Qualifications:

• Bachelors degree with 5 years or a Masters degree with 2 years of experience in molecular biology pharmaceutical sciences or a related field.
  
• Molecular Biology: Required proficiency in advanced cloning (Gibson assembly Golden Gate site-directed mutagenesis) and IVT.
  
• Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.
  
• Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g. T cells monocytes hepatocytes).
  
• Desired proficiency in Snapgene Prism and MS Office.
  

If interested please send us your updated resume at

/