Research Associate II, Product Development

AskBio Inc. a wholly owned and independently operated subsidiary of Bayer AG is a fully integrated gene therapy company dedicated to developing life-saving medicines and changing lives. The company maintains a portfolio of clinical programs across a range of neuromuscular central nervous system cardiovascular and metabolic disease indications with a clinical-stage pipeline that includes investigational therapeutics for congestive heart failure limb-girdle muscular dystrophy multiple system atrophy Parkinsons disease and Pompe disease. AskBios gene therapy platform includes Pro10 an industry-leading proprietary cell line manufacturing process and an extensive array of capsids and promoters. With global headquarters in Research Triangle Park North Carolina and European headquarters in Edinburgh Scotland the company has generated hundreds of proprietary capsids and promoters several of which have entered pre-clinical and clinical testing.

Our vision: Pioneering science to create transformative molecular medicines.

Our mission: Lead innovative science and drive clinical outcomes to transform peoples lives.

Our principles:

• Advance innovative science by pushing boundaries.

• Bring transformative therapeutics to patients in need.

• Provide an environment for employees to reach their fullest potential.

Our values:

• Be a Pioneer. We are not afraid of the impossible and to innovate to make gene therapies accessible to those in need.

• Cultivate Collaboration. Strive to be the best teammate actively listen openly communicate and embrace diverse points of view.

• Embrace Responsibility. We are humbled by the enormity of our hold a relentless commitment to advance science and clinical outcomes for our patients families and caregivers.

• Raise the Bar. Continuously drive improvements and efficiencies. Seek and provide constructive feedback. Have a bias for learning and action.

• Act with Uncompromising Integrity. Be honest transparent and committed to doing whats right in every situation. Make clear commitments and follow through.

Position Summary

The Research Associate II is a technical role utilizing a broad range of experimental techniques to support platform therapeutic pipelines and R&D activities with a primary focus on early cardiovascular programs. The RA II will perform optimization development and data delivery with increased independence supporting key preclinical studies in gene therapy R&D.

The ideal candidate will have:

• Direct expertise with mammalian cell culture including primary and immortalized cell systems and strong aseptic technique

• Direct experience with quantitative molecular biology techniques including isolation of DNA RNA and protein from cells and tissues; quantitative PCR and RTPCR (preference for direct experience with ddPCR); ELISA; SDSPAGE; and Western blot/automated Western (e.g. Jess)

• Strong data collection documentation and reporting skills with the ability to work independently and as part of a research team

This position is office-based based in RTP NC and reports to the Senior Scientist Product Development.

Job Responsibilities

• Support scientists in the design planning and execution of experiments to analyze protein RNA and DNA from in vitro and in vivo studies contributing to key studies in gene therapy R&D

• Perform a broad range of experimental techniques independently (e.g. cell culture nucleic acid and protein extraction qPCR/RT-qPCR/ddPCR ELISA SDS-PAGE Western blot/Jess and related assays) to support platform and therapeutic programs

• Process track and analyze large numbers of samples in parallel while maintaining high quality and consistency in assay execution

• Maintain accurate up to date records of all experimental work in electronic lab notebooks; support maintenance of databases sample inventories and reagent inventories as needed

• Assist in the development and optimization of experimental protocols; contribute to assay and workflow improvements

• Generate high quality reproducible data and contribute to data analysis interpretation and presentation

• Contribute to and/or author study reports and study protocols

• Ensure laboratory areas are clean safe and well organized; monitor and restock common reagents and supplies; support proper operation and routine maintenance of equipment; and adhere to all safety and compliance guidelines

• Help coach and support peers within the workgroup (e.g. sharing best practices cross training on internal assays) to enable effective day-to-day workflow

Minimum Requirements

• MS in Molecular Biology Biochemistry or related field; or BS degree in Molecular Biology Biochemistry or related field with 2 years relevant laboratory experience; or High School Diploma with 6 years relevant laboratory experience

• Hands-on experience with molecular biology techniques including DNA/RNA isolation SDS-PAGE qPCR/ddPCR and Western blot

• Prior experience with mammalian cell culture and aseptic technique

• Ability to follow established protocols with minimal supervision and deliver reliable reproducible results

Preferred Education Experience and Skills

• Experience in NGS library production and sequencing is highly desired

• Comfort working across diverse experimental workflows and learning new techniques as project needs evolve

AskBio Inc. (AskBio) is an Equal Opportunity Employer and does not discriminate against any employee or applicant for employment because of race color religion gender sexual orientation gender identity national origin age disability veteran status or any other protected status prohibited under Federal State or local laws. All employment decisions are based on valid jobrelated requirements. If you are a qualified individual with a disability or a disabled veteran and are unable or limited in your ability to use or access our website you may request a reasonable accommodation to express interest in a specific opening by calling us at or sending us an email at .

Agencies: Please do not contact any employee at AskBio about this requisition. Any resume submitted by a recruitment agency to any employee at AskBio through any medium will be deemed the sole property of AskBio unless the agency was engaged by AskBio Talent Acquisition team to recruit for that position. All agencies must have a prior executed service agreement with AskBio prior to any search engagement. If a candidate who was submitted outside of the AskBio agency process is hired by AskBio no fee or payment of any kind will be paid to the agency.