Principal Scientist, Animal Genetic Engineering Antibody Discovery

At Johnson & Johnsonwe believe health is everything. Our strength in healthcare innovation empowers us to build aworld where complex diseases are prevented treated and curedwhere treatments are smarter and less invasive andsolutions are our expertise in Innovative Medicine and MedTech we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow and profoundly impact health for more at

As guided by Our Credo Johnson & Johnson is responsible to our employees who work with us throughout the world. We provide an inclusive work environment where each person is considered as an individual. At Johnson & Johnson we respect the diversity and dignity of our employees and recognize their merit.

Job Function:

Discovery & Pre-Clinical/Clinical Development

Job Sub Function:

Biotherapeutics R&D

Job Category:

Scientific/Technology

All Job Posting Locations:

Cambridge Massachusetts United States of America Spring House Pennsylvania United States of America

Job Description:

At Johnson & Johnson we believe health is everything. Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented treated and cured where treatments are smarter and less invasive and solutions are personal.

Through our expertise in Innovative Medicine and MedTech we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow and profoundly impact health for humanity. Learn more at.

As guided by Our Credo Johnson & Johnson is responsible to our employees who work with us throughout the world. We provide an inclusive work environment where each person is considered as an individual. At Johnson & Johnson we respect the diversity and dignity of our employees and recognize their merit.

We are searching for the best talent for a Principal Scientist Animal Genetic Engineering Antibody Discovery to be based in Cambridge MA or Spring House PA.

Purpose:

We are seeking an innovative hands-on Principal Scientist to design and engineer wild-type or transgenic mouse models that enhance the efficiency and success rate of antibody discovery against historically challenging target classes including multi-pass membrane proteins and G protein-coupled receptors (GPCRs). This role sits at the intersection of mouse genetic engineering B cell biology and therapeutic antibody discovery and contributes across the full arc of in vivo model innovation from concept and proposal through externally executed engineering to in-house validation within our antibody discovery platform.

This role will combineexpertiseinmouse genetics CRISPR genome engineering B cell immunology and antibody discoverytoestablishnext-generation platforms for challenging therapeutic targets where conventional antigen-baitapproaches are limited.A deep understanding of B cell biology and the humoral immune response is central to this position. The successful candidate will apply that expertise to rationally engineer the mouse humoral system for example to broaden the antibody repertoire break tolerance to conserved self-antigens and improve the quality and diversity of B cell responses against difficult antigens enables B cell identification without antigen labeling and recover conformational and membrane-dependent binders often missed by bait-based sorting. They will identify opportunities build the scientific rationale draft detailed engineering and study plans and secure endorsement from leadership. They will then partner with Contract Research Organizations (CROs) to execute the animal engineering work and subsequently test and validate the engineered animals in end-to-end antibody discovery campaigns.

Responsibilities:

• Strategy & Proposal Development: Identify and prioritize opportunities where genetic engineering of wild-type or transgenic mouse models can improve antibody discovery outcomes enabling B cell identification without antigen labeling for difficult targets such as membrane proteins GPCRs and other low-immunogenicity or highly conserved antigens. Develop the scientific rationale draft detailed engineering and study plans and secure endorsement from leadership and relevant governance bodies.

• Animal Engineering via CRO Partnership: Partner with and scientifically manage external CROs to execute animal engineering work including targeting/construct design genome editing (e.g. CRISPR/Cas9) founder generation and colony establishment. Serve as the primary scientific and technical point of contact ensuring deliverables meet quality standards timelines and design intent.

• Testing & Validation: Design and execute studies to test and validate engineered animals within the antibody discovery workflow enabling B cell identification without antigen labeling and benchmark activation-based sorting against conventional antigen-bait approaches using diversity affinity specificity functionality and developability metrics.

• Technical Innovation & B Cell Engineering: Stay at the forefront of transgenic and genome-engineering technologies B cell biology and in vivo antibody discovery. Evaluate and integrate new approaches (e.g. humanized immunoglobulin loci alternative genetic backgrounds and tolerance-breaking or antigen-presentation strategies) that shape B cell development repertoire and the germinal-center response to overcome the challenges associated with recoveries of conformational and membrane-dependent binders often missed by bait-based sorting.

• Cross-Functional Portfolio Support: Lead the design of immunogens and immunization strategies evaluate germinal center responses and assess immune response quality to drive successful antibody discovery campaigns.

• Cross-Functional Collaboration: Work closely with antibody discovery protein sciences immunology and therapeutic area teams to ensure model design aligns with downstream discovery developability and pharmacology needs.

• Data Analysis & Communication: Rigorously analyze and interpret data maintain high-quality records and clearly communicate strategy progress and results to leadership and stakeholders to enable data-driven decisions.

• Compliance & Animal Welfare: Ensure all in vivo work internal and at CROs is designed and conducted in accordance with IACUC requirements applicable regulations and the highest standards of animal welfare and the 3Rs.

• External Engagement: Monitor the external landscape evaluate emerging technologies and potential partners/CROs and represent the company in relevant scientific interactions to bring innovation into the organization.

Qualifications:

• Ph.D. in a relevant scientific discipline (e.g. Immunology Molecular Biology Genetics Developmental Biology Biochemistry or Bioengineering) typically with 3 years of relevant post-graduate experience in academia and/or industry.

• StrongexpertiseinB cell biology including germinal center responses class switching antibody affinity maturation and class switching immunoglobulin genetics and immune tolerance memory B cell biology and plasma cell differentiation.

• Demonstrated hands-on expertise in mouse genetic engineering including one or more of the following: transgenesis CRISPR/Cas9 genome editing knock-in/knock-out generation humanized model design targeting-vector/construct design and colony breeding/management.

• Experience with antibody discovery workflows including immunization and immunogen strategy in vivo immune responses and hybridoma B-cell or single-B-cell technologies. Direct experience of antibody discovery against challenging targets (membrane proteins GPCRs ion channels) is highly desirable.

• Hands-on proficiency with immune-characterization methods such as flow cytometry/FACS B cell sorting ELISA and ELISpot.

• Experience designing scientific proposals and study plans and gaining alignment or endorsement from senior scientists leadership or governance bodies.

• Experience managing or collaborating with external partners/CROs for in vivo or engineering work including oversight of quality timelines and deliverables is preferred.

• Understanding of IACUC processes animal welfare regulations and the 3Rs.

• Strong problem-solving experimental design and data-analysis skills with the ability to work independently and drive projects to completion.

Leadership requirements

• Independently lead platform development programs from concept through implementation.

• Develop scientific strategy experimental plans and validation frameworks.

• Communicate findings and recommendations to cross-functional teams and leadership.

• Mentor junior scientists and contribute to a highly collaborative research environment.

• Translate emerging technologies and scientific advances into scalable discovery platforms.

Johnson & Johnson is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race color religion sex sexual orientation gender identity age national origin disability protected veteran status or other characteristics protected by federal state or local law. We actively seek qualified candidates who are protected veterans and individuals with disabilities as defined under VEVRAA and Section 503 of the Rehabilitation Act.

Johnson and Johnson is committed to providing an interview process that is inclusive of our applicants needs. If you are an individual with a disability and would like to request an accommodation please email the Employee Health Support Center () or contact AskGS to be directed to your accommodation resource.

Required Skills:

Preferred Skills:

Analytical Reasoning Biochemistry Biotechnology Chemistry Manufacturing and Control (CMC) Clinical Research and Regulations Clinical Trial Designs Coaching Critical Thinking Drug Discovery Development Emerging Technologies Molecular Diagnostics Organizing Pharmacovigilance Presentation Design Process Improvements Productivity Planning Scientific Research

The anticipated base pay range for this position is :

$117000.00 - $201250.00

Additional Description for Pay Transparency:

Subject to the terms of their respective plans employees are eligible to participate in the Companys consolidated retirement plan (pension) and savings plan (401(k)).



This position is eligible to participate in the Companys long-term incentive program.



Subject to the terms of their respective policies and date of hire employees are eligible for the following time off benefits:

Vacation 120 hours per calendar year

Sick time - 40 hours per calendar year; for employees who reside in the State of Colorado 48 hours per calendar year; for employees who reside in the State of Washington 56 hours per calendar year

Holiday pay including Floating Holidays 13 days per calendar year

Work Personal and Family Time - up to 40 hours per calendar year

Parental Leave 480 hours within one year of the birth/adoption/foster care of a child

Bereavement Leave 240 hours for an immediate family member: 40 hours for an extended family member per calendar year

Caregiver Leave 80 hours in a 52-week rolling period10 days

Volunteer Leave 32 hours per calendar year

Military Spouse Time-Off 80 hours per calendar year

For additional general information on Company benefits please go to: - Experience:

Staff IC