Cytogenetics Technologist
Job Location:
Redwood City, CA - USA
Yearly Salary:
Posted on:
Vacancies:
Job Summary
Retro develops therapies for diseases driven by the biology of aging. We focus on cellular reprogramming and autophagy to rejuvenate cell and tissue function with the ultimate aim of adding 10 years to healthy human lifespan.
Were building a mission-driven team of accomplished and kind individuals who embrace our startup culture of rapid iteration transparency and versatility.
We are hiring a new member of the HSC team who will help bring G-banding and karyotyping in-house. Youll stand up this capability and enable our team to get faster more cost-effective reads on the genomic stability of our cell products. You will be the primary internal owner of this capability and should be comfortable establishing and validating the workflow with limited day-to-day cytogenetics supervision. Your primary focus will be establishing and running the cytogenetics assay but youll also help maintain our iPSC cultures the starting material the assay depends on.
About you:
Youre a careful hands-on technologist who takes pride in high-quality chromosome preparations and confident karyotype calls. You are comfortable bringing an established cytogenetics workflow into a new laboratory environmentsetting up the process confirming that it performs consistently documenting it clearly and troubleshooting issues as they arise. Youre at home at the microscope and equally happy in a tissue-culture hood keeping iPSC lines healthy. Youre collaborative easy-going proactive in how you communicate and flexible about scheduling when the culture rotation calls for it.
What youll do in this role:
Transfer in implement and document an established G-banding and karyotyping workflow in-house for HSC program cell products.
Execute the full cytogenetics workflow (initially in research/PD and then in our GMP suite): metaphase arrest cell harvest hypotonic treatment slide preparation fixation Giemsa staining metaphase imaging and software-assisted karyotype analysis.
Compare initial in-house results against historical data and document appropriate operating and quality-control criteria.
Analyze metaphase spreads to identify numerical and structural chromosomal abnormalities and document findings clearly.
Support genomic-stability assessment of human primary cells
Culture passage expand bank and prepare iPSC samples in preparation for karyotyping
Track clone-level genomic stability passage number culture conditions and QC status across lines.
Coordinate with CROs shipments of samples for genomic instability analysis
Maintain organized records sample tracking and imaging files and develop SOPs for the workflows you build.
You might thrive in this role if you:
Have several years of hands-on G-banding and karyotype analysis experience with demonstrated ability to make independent chromosome assessments and troubleshoot chromosome preparation and image quality.
Have experience with imaging software such as MetaSystems / Ikaros or Cytovision.
Have hands-on mammalian cell culture experience with strong sterile technique.
Are comfortable with high-magnification microscopy.
Are highly organized and able to run multiple clone/sample workflows in parallel.
Are available for weekend work as part of a team rotation.
Bonus if you:
Have experience standing up or validating a new assay in-house.
Hold current CG(ASCP) certification in cytogenetics.
Hold a Bachelors or Masters degree in cell biology molecular biology cytogenetics genetics stem cell biology biomedical sciences or a related field.
Have hands-on experience maintaining iPSC cultures.
At Retro we dont use titles. New hires join as a Member of X Team. The expectations for this role are generally aligned to the level of Cytogenetics Technologist.
Total compensation also includes generous equity and benefits including:
Medical dental and vision insurance for you and your family
401(k) plan with 4% matching
Unlimited time off and 10 company holidays per year
Annual learning & development stipend
Free lunch and snacks
We take pride in cultivating an environment that fosters collaboration open communication and authenticity. We are an equal opportunity employer; we do not discriminate on the basis of race religion color national origin gender sexual orientation age marital status veteran status or disability status.
Required Experience:
IC
About Company
We take pride in cultivating an environment that fosters collaboration, open communication, and authenticity. We believe that great results are best delivered by a highly creative team working in concert. We are an equal opportunity employer; we do not discriminate on the basis of rac ... View more
