Associate Researcher I (Dr Yang's Lab) Neurosciences

The Associate Researcher I will support research projects focused on stem cell disease modeling gene engineering and molecular/cellular biology. The individual will work closely with laboratory investigators and trainees to perform routine and advanced experimental procedures maintain laboratory operations and assist with data collection and analysis.

• Maintenance expansion quality control and differentiation of human induced pluripotent stem cells (iPSCs) and neuronal cultures.
• Performing gene engineering experiments including CRISPR/Cas9 genome editing cloning transfection lentiviral production and validation of engineered cell lines.
• Conducting molecular biology experiments including RNA extraction qPCR PCR western blotting immunocytochemistry and protein/RNA analyses.
• Performing cellular and biochemical assays to characterize disease phenotypes and evaluate experimental outcomes.
• Assisting with experimental design data collection record keeping and analysis of research results.
• Preparing reagents maintaining laboratory inventories ordering supplies and ensuring proper laboratory organization and compliance with institutional safety protocols.
• Maintaining accurate laboratory notebooks and experimental records.
• Assisting in optimization and troubleshooting of experimental protocols.
• Collaborating with other laboratory personnel on ongoing research projects and contributing to general laboratory operations.
• Performs other related duties

• Bachelors or Masters degree in science or related field (Biology Neuroscience Molecular Biology Cell Biology Biomedical Sciences or a related scientific discipline) required
• No experience required. One year of research experience preferred.
• Experience with human iPSC culture neuronal differentiation and stem cell-based disease modeling.
• Experience with CRISPR/Cas9 genome engineering cloning and lentiviral techniques.
• Familiarity with molecular and biochemical techniques including PCR qPCR western blotting immunocytochemistry and protein/RNA analyses.
• Experience with fluorescence microscopy and image analysis software is preferred.
• Strong quantitative and analytical skills with ability to interpret experimental data.