Assistant Scientist-Proteomics

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The University of Miami/UHealth Department of SCCC has an exciting opportunity for a Full Time Assistant Scientist to work at the UHealth campus in Miami Fl.

CORE JOB SUMMARY
The Assistant Scientist carries out a particular research agenda in support of the Universitys research goals and objectives. Moreover the Assistant Scientist SOM contributes to the scientific community by publishing
research findings and promotes the Universitys reputation as a leader in the field.

CORE JOB FUNCTIONS

• Contributes to basic and applied research activities and authors scientific publications technical and agency reports and patent preparations.

• Collaborates with senior staff to publish findings in refereed journals.

• Creates and conducts experiments and analyzes results.

• Works with other researchers to use and develop end products.

• Writes extramural proposals with approval from appropriate senior staff.

• Provides ready access to all experimental data for managing staff.

• Requests or acquires equipment and supplies for assigned project(s).

• Uses the Internet surveys legal documents and other available sources to collect information.

• Applies for grants to continue research and prepares materials for submission to granting agencies and foundations.

• Adheres to University and unitlevel policies and procedures and safeguards University assets.

This list of duties and responsibilities is not intended to be allinclusive and may be expanded to include other duties or responsibilities as necessary.

CORE QUALIFICATIONS
Education: Ph.D. M.D. or other doctoral level degree required

Experience: Minimum 1 year of relevant experience

Knowledge Skills and Attitudes:
Skill in collecting organizing and analyzing data.
Ability to recognize analyze and solve a variety of problems.
Ability to exercise sound judgment in making critical decisions

The Sylvester Comprehensive Cancer Center is looking for an Asst. Scientist to join the newly established Proteomic Shared Resources to apply state of the art LC-MS based methods and techniques for quantitative proteomics.

As an Asst. Scientist you will be responsible for the maintenance and operation of a modern high resolution orbitrap mass spectrometer as well as performing detailed biochemical sample processing addition you will be part of a small agile interdisciplinary team that seeks to invent innovative methods that can be used in the development of diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology. The individual will be:

• Primary responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument for proteomic applications.
• Will manage inventory of lab consumables including ordering receiving and storage of supply stocks.
• Prepare protein samples from a wide range of specimen (e.g. cells tissues biofluids and SDS Page gels)
• Implement of both bottom-up & top-down proteomic approaches.

• Responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and mass bar-codes. Detection of post-translational modifications (e.g. phosphorylation acetylation).
• Perform relative quantification of proteins by label-free and stable-isotope labelling strategies (e.g. SILAC TMT).
• Responsible for structural proteomics (e.g. cross-linking pull-down experiments).
• Will conduct Single cell proteomics analysis.
• Intact protein analysis & MW determination.
• Use of proteomics software (e.g. Proteome Discoverer MASCOT) for data analysis.
• Responsible for operation maintenance and troubleshooting of Orbitrap Ascend Tribrid Mass Spectrometry and other laboratory equipment as needed.
• Assesses experimental results and troubleshoot methods and instruments to optimize research protocols.
• Collaborates efficiently with technicians graduate students post-docs PIs the laboratory director & colleagues.

Education:

• Ph.D. in Science or Engineering (Biochemistry Bioengineering Biology Chemistry or related field)

Knowledge Skills and Attitudes:

• 5 years of related experience with mass spectrometry instruments which includes 2 years of experience in proteomics mass spectrometry applications.
• Hands-on experience operating and/or maintaining LC-MS instruments and supporting equipment (including auxiliary systems such as chillers controls electronics pumps vacuum systems etc.)
• Strong interest in handling processing and analyzing high-throughput datasets.
• Motivation to constantly develop skills and expertise.
• Ability to work both independently and as a team member while engaging in the organization of the proteomics core.
• Excellent analytical communication problem solving and organizational skills.
• excellent written and oral communication skills.
• Established track record of publications and demonstrated ability to complete projects.
• Capability to learn complex tasks with instruction and to use experience to perform a variety of new techniques.

Preference will be given.

• expertise in one or more of the following technical areas: quantitative proteomics single cell proteomic analysis global proteome profiling.
• Previous hands-on operation of Thermo Tribrid Orbitrap LC-MS Systems (e.g. Fusion Eclipse Ascend).

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The University of Miami offers competitive salaries and a comprehensive benefits package including medical dental tuition remission and more.

UHealth-University of Miami Health System South Floridas only university-based health system provides leading-edge patient care powered by the ground breaking research and medical education at the Miller School of Medicine. As an academic medical center we are proud to serve South Florida Latin America and the Caribbean. Our physicians represent more than 100 specialties and sub-specialties and have more than one million patient encounters each year. Our tradition of excellence has earned worldwide recognition for outstanding teaching research and patient care. Were the challenge youve been looking for.

The University of Miami is an Equal Opportunity Employer. Applicants and employees are protected from discrimination based on certain categories protected by Federal law.

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