Research Associate
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Job Location:
Basel - Switzerland
Monthly Salary:
Not Disclosed
Posted on:
18 hours ago
Vacancies:
1 Vacancy
Job Summary
The Botnar Institute of Immune Engineering (BIIE) is a newly founded non-profit research organization aiming to become a world leading research institute for the advanced study of immunological systems with a mission to develop translational solutions for the diagnosis treatment and prevention of disease. The scientific foundation of the BIIE and the broader field of Immune Engineering is based on the intersection of three interdisciplinary subdomains: (i) systems immunology (ii) synthetic immunology and (iii) computational immunology. The BIIE will serve as a hub for multidisciplinary science by uniting people with diverse expertise in immunology biomedicine bioengineering systems and synthetic biology computational biology artificial intelligence and machine learning. As part of the mission of BIIE to make a lasting impact it will dedicate significant efforts in training early-stage researchers in this unique multidisciplinary environment thus enabling them to become future leaders in Immune Engineering.
We are seeking a Research Associate to join our new Biobetters translational initiative at the Botnar Institute of Immune Engineering where they will support high-throughput experimental workflows in antibody lead optimization and characterization. As such you will support us on generating and screening large libraries in yeast high-throughput DNA sequencing transient protein production and purification from mammalian cells as well as a range of antibody characterization methods.
Tasks
- High-Throughput Production and Purification: Execute cloning high-throughput mammalian transfection workflows (CHO/HEK) & purification of 100s of antibody variants.
- Biophysical Characterization: Perform critical developability assays including UPLC-SEC Nano-DSF (thermal stability) and PS-SINS.
- Plate-based Assays: Perform assets to quantify potency and polyreactivity of antibody candidates (e.g. cell-based ELISA).
- Large Library Screening: construct and screen yeast display libraries using FACS and MACS. Prepare samples and run NGS (Illumina MiSeq).
- Data Management: Maintain rigorous electronic records using Benchling ensuring high-quality data integrity for our computational pipelines.
- Continuous Improvement: Collaborate with the team to optimize screening protocols and troubleshoot hardware/assay deviations.
Requirements
- Educational Background: BSc or MSc in Biochemistry Biotechnology Molecular Biology or a related field.
- Technical Foundation: Hands-on experience with microbial and mammalian cell culture molecular biology (PCR/Cloning) and protein purification.
- Comfortable with software-driven workflows and electronic lab notebooks (Benchling experience is a strong asset).
- Strong organizational skills for managing large sets of clones samples and experimental data.
- Strong communication in English spoken and written.
Preferred Qualifications
- Experience operating liquid handling platforms (e.g. Hamilton Analytik Jena or Opentrons).
- Hands-on experience with:
- UPLC/HPLC systems or other biophysical stability assays.
- Flow cytometry.
- Illumina MiSeq sample preparation.
At BIIE you will be an integral part of a mission to accelerate immunology breakthroughs. This role offers the opportunity to shape how cutting-edge research data is captured and utilized by building a digital foundation for our labs. You will work in a highly collaborative and interdisciplinary setting. We offer a competitive salary and benefits package opportunities for professional development (including Benchling certification programs) and the chance to make a tangible impact on scientific discovery by empowering researchers with effective technology.
Key Skills
- Laboratory Experience
- ELISA
- Immunoassays
- Mammalian Cell Culture
- Biochemistry
- Cell Biology
- Research Experience
- Cell Culture
- Molecular Biology
- Microscopy
- Research Laboratory Experience
- Western Blot
About Company
The Botnar Institute of Immune Engineering is a newly founded non-profit research organization aiming to become a world leading research institute for the advanced study of immunological systems with a mission to develop translational solutions for the diagnosis, treatment and prevent ... View more
