Senior Research Associate, Reagents (Oligo Chemistry)
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Job Location:
Berkeley, CA - USA
Monthly Salary:
$ 82000 - 117875
Posted on:
17 hours ago
Vacancies:
1 Vacancy
Job Summary
About Glyphic:
At Glyphic Biotechnologies we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date we have raised >$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing.
What we are looking for in you
We are seeking a highly motivated and collaborative chemist for the position of Senior Research Associate. Reporting to the Senior Scientist you will work alongside a team of multidisciplinary scientists to execute and develop processes for the synthesis purification and characterization of oligonucleotides at scale enabling Glyphics mission of developing next-generation single-molecule protein sequencing assays. This is a full-time exempt in-person position with work conducted at our Berkeley CA location.
As a Senior Research Associate you will support the project lead in operating automated DNA synthesizers and purification systems working closely with Oligonucleotide scientists to develop and execute oligonucleotide synthesis across a range of scales. The ideal candidate has a strong foundation in synthetic organic chemistry and the ability to independently execute complex multi-step oligonucleotide synthesis and purification workflows with a high level of precision and reliability.
In this role you will have the unique opportunity to directly contribute to highly innovative projects at the intersection of synthetic chemistry molecular biology and biophysics.
What youll do
- Collaborate closely with the project lead to execute daily oligonucleotide synthesis and purification requests using automated systems.
- Independently synthesize oligonucleotides at 200 nmol to >20 umol starting scales using MerMade 12 KnA H2 etc. and troubleshoot instrument issues.
- Actively propose ideas for new reagents and/or methods to reduce impurities such as deletions alkylations synthesized oligos and resolve commonly observed problems in solid-phase oligo synthesis.
- Routinely handle moisture sensitive reagents such as phosphoramidites acid anhydrides dry solvents etc. and take necessary precautions to ensure high quality of reagents during synthesis.
- Develop and execute methods for purification of oligonucleotides to achieve n-1 resolution at semi-prep scale.
- Engage with the broader scientific team in discussions about oligo design and court ideas for expanding Glyphics oligonucleotide toolbox at all times.
- Effectively prioritize urgent synthesis requests to meet critical timelines.
- Provide routine updates to the project lead and the Reagents team in presentation form.
What you need
:
- BS/MS in Chemistry or Chemical Engineering with 2-3 years of experience in a synthetic chemistry lab.
- Hands-on experience with solid phase oligonucleotide synthesis and characterization (e.g. anion exchange/FPLC HPLC).
- Hands-on experience with moisture sensitive chemical reactions (e.g. phosphoramidite chemistry).
- Familiarity with fundamental concepts of nucleotide chemistry nucleophilic displacement reactions and biochemistry of DNA.
- Experience with purification of polar and/or non-polar compounds using at least one purification technique (e.g. RP-HPLC Normal phase HPLC HILIC Size Exclusion Chromatography).
- Legal authorization to work in the United States on a full-time basis. Visa sponsorship is not available for this role.
- Located in or able to relocate to the San Francisco Bay Area and available to start within two weeks of offer acceptance.
Nice to have:
- Hands-on experience operating LGC MerMade oligonucleotide synthesizers.
- Experience with LC-MS analysis of oligonucleotides and/or proteins.
- Ability to understand and resolve characterization outputs.
- Experience with bioconjugation reactions with DNA such as amide coupling Cu-click copper free click chemistry etc.
Were looking for a teammate that:
- Navigates complex team dynamics partnerships and challenges with creativity and logic.
- Operates with adaptability urgency and flexibility in evolving environments thriving in ambiguity.
- Drives work forward without needing to be asked taking responsibility for outcomes rather than tasks.
- Treats obstacles as problems to be creatively solved not reasons something cant be done.
- Applies sound judgment to the best available information testing learning and iterating.
- Shares early and directly when assumptions change results are unclear or timelines are at risk.
What you can expect from this role
Work environment:
- Collaborative culture where your ideas and expertise are valued
- Direct impact on product development and company direction
Professional growth:
- Learn from a diverse team of world-class scientists and engineers
- Contribute to first-of-their kind technologies high-impact publications and patents
Compensation
Estimated Base Salary $82000 - $117875
This is the pay range for this position that we reasonably expect to pay. Individual compensation is based on various factors including experience education skillset and geographic location. This range is for the SF Bay Area California location and may be adjusted to the labor market in other geographic areas.
Benefits and Perks:
- Employee Stock Option Plan
- 100% Health Plan Coverage for Employees & Dependents (Medical Dental & Vision)
- Employer Retirement Contributions to 401(k)
- Generous Paid Time Off
- Paid Maternity and Paternity Leave
- Health & Wellbeing Program
- Office Snacks and Beverages
- Regular Team Bonding Activities
We are an Equal Opportunity Employer. We celebrate diversity and are committed to creating an inclusive environment for all employees. Individuals seeking employment at Glyphic Biotechnologies are considered without regard to race color religion national origin age sex marital status ancestry physical or mental disability veteran status gender identity or sexual orientation.
Required Experience:
Senior IC
Key Skills
- Laboratory Experience
- Immunoassays
- Mammalian Cell Culture
- PCR
- Biochemistry
- Assays
- Cell Biology
- Research Experience
- Cell Culture
- Molecular Biology
- Flow Cytometry
- Western Blot
About Company
We sequence proteins. We have exciting updates coming soon... Contact us or sign up for updates below to be the first to know. Let's Chat. Partner. Collaborate. Team Up. Discuss. Talk. Co-Develop. Let's build something great and change the future of proteomics together. Send us a mess ... View more
