Principal Scientist, Molecular Biology

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About Innovative Medicine:

Our expertise in Innovative Medicine is informed and inspired by patients whose insights fuel our science-based advancements. Visionaries like you work on teams that save lives by developing the medicines of tomorrow.

Join us in developing treatments finding cures and pioneering the path from lab to life while championing patients every step of the way.

Learn more at are searching for the best talent for a Principal Scientist Molecular Biology to be onsite in Spring House PA.

Purpose:
The in Vivo Antibody Discovery group within Biologics Discovery is responsible for lead antibody generation from immunized animals with state-of-the-art technologies including single B cell sorting hybridoma display and this key role the Principal Scientist will lead a team responsible for recovery of antibody sequences from selected hybridoma cells or single B cells. The candidate is expected to work with automation experts to automate NGS workflow for efficient sequence -depth knowledge and extensive experience in molecular cloning of BCR using various approaches including RT-PCR Topo cloning in-fusion cloning multiplex NGS 10X genomics are required. A strong understanding of B-cell recovery methods such as Hybridoma technology and primary B-cell cloning will also be important. This is a critical function in a multifaceted Biologics Discovery organization committed to bringing the next generation of biotherapeutic molecules to patients. We are looking for a motivated candidate with scientific and technical expertise who can collaborate across discovery areas to bring value to our portfolio of innovative drug concepts. Proficiency to work within a highly coordinated fast-paced environment will be a significant attribute. If this sounds like you come join our team!

You will be responsible for:

• Provide day-to-day in-person guidance and mentoring within the V-Gene Recovery (VGR) team
• Lead antibody sequence recovery from selected hits of diverse sources including hybridoma single B cells immune repertoires and in vitro display platforms.
• Execute and optimize molecular biology workflows including Next-Generation Sequencing (NGS) and conventional Sanger sequencing to enable efficient and accurate antibody discovery.
• Work with automation experts to automate and streamline NGS workflows identifying opportunities for process improvements to enhance sequencing accuracy and efficiency.
• Manage laboratory operations by maintaining equipment ensuring sufficient inventory of supplies and overseeing the routine maintenance of NGS instruments and related laboratory equipment.
• Conduct comprehensive data analysis interpret sequencing results and generate detailed reports. Bioinformatics experience is a plus.
• Present research findings in internal meetings clearly communicating scientific results and providing insights into data interpretation.
• Collaborate with multidisciplinary teams across functional areas to advance therapeutic programs contributing scientific expertise to multiple projects in a dynamic and fast-paced environment.
• Document work accurately and promptly using Electronic Lab Notebook systems (e.g. Signal) and generate protocols and SOPs.
• Work closely with stakeholders and project managers to address operational needs and ensure seamless workflow execution.
• Demonstrate scientific curiosity and problem-solving skills contributing to innovation and continuous improvement in molecular biology and sequencing workflows.

Qualifications / Requirements:

Education and Years of Experience:

• Minimum of a Bachelors degree is required
• PhD in Molecular Biology Biomedical Engineering Biochemistry or a related field is highly preferred

Required:

• Minimum 6 years of experience in the pharmaceutical industry and/or academic setting
• Team management experience
• Proficiency in molecular biology techniques including PCR molecular cloning and plasmid design.
• Expertise with DNA sequencing (NGS 10x Genomics Sanger) and sequence data analysis related to antibody discovery.

Preferred:

• Familiarity with techniques for antibody repertoire analysis and single cell analysis.
• Knowledge of data management and visualization tools for large datasets and knowledge with statistical analysis of NGS results.
• Knowledge on leveraging repertoire sequencing from immunized animals for AI/ML-driven antibody discovery

Johnson & Johnson is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race color religion sex sexual orientation gender identity age national origin disability protected veteran status or other characteristics protected by federal state or local law. We actively seek qualified candidates who are protected veterans and individuals with disabilities as defined under VEVRAA and Section 503 of the Rehabilitation Act.

Johnson & Johnson is committed to providing an interview process that is inclusive of our applicants needs. If you are an individual with a disability and would like to request an accommodation external applicants please contact us via internal employees contact AskGS to be directed to your accommodation resource.

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