Research Lab Tech Lead

Site: The General Hospital Corporation

Mass General Brigham relies on a wide range of professionals including doctors nurses business people tech experts researchers and systems analysts to advance our mission. As a not-for-profit we support patient care research teaching and community service striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.

Job Summary

We are seeking an experienced Research Technologist to lead our noninvasive fetal sequencing project (NIFS). Standard noninvasive prenatal testing (NIPT) relies on cell-free DNA (cfDNA) analysis from maternal plasma but is restricted to detecting common aneuploidies and some microdeletions. We have developed a high-resolution noninvasive fetal sequencing approach that can detect clinically significant fetal variants with comparable accuracy to NIPT eliminating the procedural risk of invasive testing.

The candidate will be responsible for optimizing our current NIFS protocol to improve our cfDNA recovery and to validate those results against the current diagnostic gold standard.

The Talkowski Laboratory in the Center for Genomic Medicine at Massachusetts General Hospital (MGH) and the Stanley Center for Psychiatric Research at the Broad Institute of MIT and Harvard are seeking a motivated enthusiastic experienced leader to join our research teams. Our group explores the impact of genomic variation on human disease with a particular focus on the relationship between genome structure and function. We are dedicated to the development and application of innovations in genome sequencing technologies and genetic diagnostics. Our research has shown that we can use NGS techniques to more fully capture prenatal.

The candidate will be expected to have experience or a high capacity to learn the following techniques:

Handling of human blood-derived samples including isolation of plasma and buffy coat under sterile and reproducible conditions.

Sample processing labeling and biobanking under rigorous quality control and contamination-prevention measures consistent with good laboratory practice (GLP) standards.

Extraction and robust handling of cell-free DNA (cfDNA) and genomic DNA (gDNA) from plasma blood and human tissues.

Next-generation sequencing (NGS) library preparation for low-input cfDNA/gDNA samples and target hybridization protocols for exome and custom sequencing panels.

Sanger sequencing gel electrophoresis PCR and qPCR-based assays for cfDNA quantification variant detection and workflow validation.

NGS library quality control and assessment including quantification and fragment size analysis.

Accurate sample tracking and documentation across all stages of processing and analysis.



Does this position require Patient Care No



Essential Functions
-Perform DNA/RNA extraction from blood samples (incl. whole blood buffy coat plasma).

-Perform other molecular genomics procedures as needed (i.e. sequencing library production target hybridization RNAseq sample archiving tissue culture etc.)

-Learn and perform any number of molecular genomics techniques including single-cell RNAseq (scRNAseq) ATACseq ChIPseq HiC and other methods

-Independently performs non-routine highly specialized experimental procedures.

-Participates in the design and modification of research protocols.

-Develops research methodologies within the parameters of experimental protocols and research objectives.

-Composes and may present sections of research reports and manuscripts.

-Performs advanced data analysis using advanced statistical techniques.

-Responsible for the oversight and coordination of lab activities including quality assurance.

-Responsible for the oversight and coordination of the scheduling of all procedures.

-Responsible for troubleshooting problems and instructing others in highly specialized techniques.

Education
Bachelors Degree Related Field of Study required

Can this role accept experience in lieu of a degree
Yes

Licenses and Credentials

Experience
Previous Research Lab Experience 3-5 years required

Knowledge Skills and Abilities
- Organize and maintain a functioning research laboratory.
- Troubleshoot scientific and non-scientific problems.
- Proficiency in Word PowerPoint Excel and Internet searches is preferred.
- Well-versed in laboratory standards and practices.
- May teach moderately difficult-to-complex lab techniques to students and research personnel.
- May provide functional guidance to lower level technical personnel.

Onsite

185 Cambridge Street

40

Regular

Day (United States of America)

Pay Range

$62004.80 - $90750.40/Annual

Grade

7

At Mass General Brigham we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive and any offer extended will take into account your skills relevant experience if applicable education certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications; however it does not encompass all elements contributing to your total compensation addition to competitive base pay we offer comprehensive benefits career advancement opportunities differentials premiums and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth. We invite you to apply and our Talent Acquisition team will provide an overview of your potential compensation and benefits package.

The General Hospital Corporation is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race color religious creed national origin sex age gender identity disability sexual orientation military service genetic information and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process to perform essential job functions and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973 the Vietnam Veterans Readjustment Act of 1974 and Title I of the Americans with Disabilities Act of 1990 applicants who require accommodation in the job application process may contact Human Resources at .

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