Job Title:Protein Expression Scientist
Job Location:Bangalore
Department:Discovery Biology
About Syngene:
Incorporated in 1993 Syngene International Ltd. is an innovation-focused global discovery development and manufacturing organization providing integrated scientific services to the pharmaceutical biotechnology nutrition animal health consumer goods and specialty chemical industries around the world. Syngene s clientele includes world leaders such as Bristol-Myers Squibb Baxter Amgen GSK Merck KGaA and Herbalife. Its innovative culture is driven by the passion of its 4240- strong team of scientists who work with clients from around the world to solve their scientific problems improve R&D productivity speed up time to market and lower the cost of innovation.
Key Responsibilities:
- Maintenance of Cell culture Storage of cells
- Independently perform experiments for protein expression-Transient transfections Stable cell line generation Insect cell expression Expression in E. coli
- Perform small scale purifications of affinity tag proteins.
- Perform analysis of proteins by SDS-PAGE Western Blot.
- Troubleshoot issues with protein expression and purification.
- Regularly prepare detailed records of experiments reports.
- Manage multiple projects simultaneously under strict deadlines.
- Help other team members in their work.
- Contribute to general lab cleanliness equipment maintenance Consumable ordering and safety.
Educational Qualification:
- MSc (Biochemistry Biotechnology or any other relevant Biological Sciences) with 9-12 years of experience in Mammalian Cell based Expression systems and microbial expression systems.
Experience:
- Experience in performing transfections in Mammalian cells in Shake flask
- Experience in performing microbial expression
- Experience with Protein analysis methods (SDS PAGE Western).
- Reasonable theoretical knowledge of Proteins Biochemistry Cell biology Mammalian Protein expression Microbial expression and affinity protein purification.
- Working knowledge of making buffers and solutions calculations of molarity dilutions measuring pH of solutions.
- Good verbal communication written communication and interpersonal skills
- Ability to manage multiple projects in parallel.
- Ability to work under strict timelines.
- Ability to properly document results and observations in timely manner
- Personal attributes organized motivated cooperative friendly respectful and communicative.
Behavioral Skills:
- Demonstrate ability to be a team player.
- Commitment to deliver the study reports within the agreed timelines
- Adaptability to changes in the dynamic lab environment
- Communicate confidently with colleagues and collaborators in the meetings and presentation of study reportsand evidence of publications in the PEER reviewed Journals
Equal Opportunity Employer:
It is the policy of Syngene to provide equal employment opportunity (EEO) to all persons regardless of age color national origin citizenship status physical or mental disability race religion creed gender sex sexual orientation gender identity and/or expression genetic information marital status status with regard to public assistance veteran status or any other characteristic protected by applicable legislation or local addition Syngene will provide reasonable accommodations for qualified individuals with disabilities.