Postdoctoral Research Associate
Postdoctoral Research Associate
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Salary Not Disclosed
1 Vacancy
Job Description
POSTDOCTORAL POSITION to study the function of novel CAR T cell-based therapies for pediatric brain tumors
St. Jude Childrens Research Hospital Memphis Tennessee USA
We are seeking a highly motivated and ambitious postdoctoral fellow to join the group of Dr. Giedre Krenciute. The lab is located in the Department of Bone Marrow Transplantation and Cellular Therapy at St. Jude Childrens Research Hospital in Memphis TN. Our lab is working on highly translational immunology research with the focus on brain tumors. We use genetically engineered human or murine T cells to design novel long-lasting and safe therapeutic approaches for pediatric brain tumors.
Major projects include:
Designing generating and optimizing chimeric antigen receptors (CARs) specific for pediatric brain tumors.
Enhancing CAR T cell effector function and longevity using different genetic manipulations
Studying brain tumor microenvironment and its effect on CAR T cell function
For representative publications please see:
Haydar D Houke H Chiang J Yi Z Od Z Caldwell K Zhu X Mercer KS Stripay JL Shaw TI Vogel P DeRenzo C Baker SJ Roussel MF Gottschalk S Krenciute G. Cell surface antigen profiling of pediatric brain tumors: B7-H3 is consistently expressed and can be targeted via local or systemic CAR T-cell delivery. Neuro Oncol. 2020
Prinzing B Schreiner P Bell M Fan Y Krenciute G* Gottschalk S*. MyD88/CD40 signaling retains CAR T cells in a less differentiated state. JCI Insight. 2020. *co-senior authors
Od Z Condori J Peterson N Zhou S Krenciute G. CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell Therapy. Cancers (Basel). 2020
Yi Z Brooke LP Cao F Gottschalk S Krenciute G. Optimizing EphA2-CAR T cells for the Adoptive Immunotherapy of Glioma. Molecular Therapy - Methods & Clinical Development. 2018
Krenciute G Prinzing BL Yi Z Wu MF Liu H Dotti G Balyasnikova IV Gottschalk S. Transgenic expression of IL15 improves antiglioma activity of IL13Ralpha2-CAR T cells but results in antigen-loss variants. Cancer Immunol Res. 2017
In addition to a Ph.D. the candidate should have expertise in the following techniques (all highly preferred but not required):
Molecular biology techniques (DNA related work (propagation in bacteria isolation PCR) RNA related work (isolation qPCR) WB CRISPR)
Cloning (ligation cloning any iteration of Gibson Assembly (InFusion NEBuilder etc)) and plasmid design using SnapGene (or equivalent software)
Tissue culture (tumor and immune cells)
Flow cytometry
Transfection transduction and transformation.
Immunology methods (cytokine detection by ELISAs and/or flow immune cell isolation from animals etc)
Animal work
Training in brain tumor biology immunology and/or CAR T cell biology is highly advantageous but not necessary.
Fluency in English is essential. Candidates should have the ability to work independently as well as in a collaborative research team and have a strong desire to make a major contribution to the field of CAR T cell biology. The fellow will benefit from the outstanding research facilities and the highly collaborative St. Jude addition the fellow will interact and work with the leaders in basic and translational immunology and brain tumor biology research within and outside the institution.
Interested applicants should send their CV a cover letter describing research experience and interests and names and email addresses of three references to:
No Search Firms
St. Jude Childrens Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.
Required Experience:
IC
Employment Type
Full-Time
