UNIV - Research Lab Technician - Hollings Cancer Center

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Job Description

Summary:

This position will operate and manage two staff-run cell sorters (FACSAria III and MoFlo Astrios EQ) five analyzers (Fortessa X-20 CytoFLEX LX and Northern Lights) 1 imaging cytometer (ImageStream mkII) and any new instrument acquired by the facility to accommodate Hollings Cancer Center and MUSC investigators. To manage the design execution and evaluation of FACS sorting and analytic flow cytometry serving as a key technical and scientific resource for the FCCS shared resource. Will be responsible for communicating experimental results to HCC investigators and assist in the preparation of manuscripts. Will actively develop novel flow cytometry-based techniques and provide cutting-edge methodology to progress the research of the HCC.

Preferred Additional Knowledge and Skills:

The ideal candidate will have advanced technical skill and theoretic understanding of FACS-based cell isolation and analytic procedures as pertaining to biomedical research with an emphasis on cancer-related experimentation. The candidate must have a proven track record of hands-on cell sorting with FACSAria and/or MoFlo Astrios cytometers and be experienced in cytometer maintenance and troubleshooting. Applicants must have a working knowledge of laboratory techniques including sample preparation for flow cytometry and experience with biohazard research. The candidate must be proficient in analysis software such as FlowJo ModFit and FACSDiva. Additional experience with Idea Elan Infinity software for laboratory scheduling and billing is desired. A bachelors degree in a scientific discipline and established history of cell sorting are strongly preferred. The position needs a current knowledge of experimental methodology and will include dedicated time for continuing education on techniques through literature review attendance of University seminars and participation at regional and/or national conferences.

Responsibilities:

35% Oversee and participate in the completion of duties shared among FCCS staff including all aspects of daily facility operation such as the maintenance troubleshooting and operation of Fortessa X-20 CytoFLEX LX Northern Lights ImageStream MkII FACSAria sorter MoFlo Astrios sorter Standard BioTools Helios/Hyperion Imaging MAss Cytometry System and any instruments purchased in the future for MUSC investigators. Assist with the technical and administrative activities of the FCCS shared resource and supervise the junior technician. Supervises the orientation and training of new users to the facility. Provides expert technical support for experiments including consultation for experimental design and analysis and ongoing troubleshooting to ensure client satisfaction

30% Works closely with principal investigators to progress their research in an efficient and timely manner. Consults with investigators regarding complex sample preparation and sorting techniques to optimize their experiments and to ensure that experiments are properly designed and competitive within the scientific community.

20% Ensures that laboratory practices including cells sorting and analytic cytometry are conducted in accordance with biosafety regulations provided by MUSC and the International Society for the Advancement of Cytometry.

10% Works closely with FCCS Director and the HCC providing statistical reports and evaluation of the scientific field to address current and future needs of the facility pertaining to both the advancement of investigator research as well as financial position of the FCCS

5% Develops and provides instructional presentations for MUSC investigators on the capability and implementation of flow cytometry in research. Presents technical advances in relevant local and national scientific meetings.

Additional Job Description

Minimum Requirements: A bachelors degree in a health-related field and one year journey-level work experience in the specialty area; or an associate degree or equivalent training in a health-related field and two years advanced work experience in the specialty area. A masters degree in a health-related field and six months entry-level experience may be substituted for the requirements stated above. Previous technical supervisory work experience is required for supervisory positions.

Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Continuous) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb stairs. (Infrequent) Ability to work indoors. (Continuous) Ability to work in all cold temperature extremes i.e. spot coolers. (Frequent) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to perform pinching operations. (Continuous) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Continuous) Ability to fully use both legs. (Continuous) Ability to reach in all directions. (Continuous) Possess good finger dexterity. (Continuous) Ability to maintain tactile sensory functions. (Continuous) Ability to maintain good olfactory sensory function. (Continuous) Ability to lift and carry 30 lbs. unassisted. (Frequent) Ability to lift objects up to 30 lbs. from floor level to height of 72 inches unassisted. (Frequent) Ability to lower objects up to 30 lbs. from height of 72 inches to floor level unassisted. (Frequent) Ability to push/pull objects up to 50 lbs. unassisted. (Infrequent) Ability to lift and carry objects up to 30 lbs. a distance of five (5) floors and to outlying buildings. (Frequent) Ability to maintain 20/40 vision corrected. (Continuous) Ability to see and recognize objects close at hand. (Continuous) Ability to see and recognize objects at a distance. (Continuous) Ability to match or discriminate between colors. (Continuous) Ability to determine distance/relationship between objects; depth perception. (Continuous) Good peripheral vision capabilities. (Continuous) Ability to maintain hearing acuity with correction. (Continuous) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to be qualified physically (by medical personnel) for respirator use initially and annually. Ability to work alone in isolated areas such as darkrooms. (Frequent) Additional New Requirements: Ability to obtain and maintain a valid drivers license. Computer literacy. Ability to work rotating shifts as required. Ability to work overtime as required. Ability to learn and use new processes tools and equipment as required.

If you like working with energetic enthusiastic individuals you will enjoy your career with us!

The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race color religion or belief age sex national origin gender identity sexual orientation disability protected veteran status family or parental status or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications merit and business need.

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