Laboratory Technologist

Job Family:

Travel Required:

Clearance Required:

What You Will Do:

We are currently searching for a laboratory technologist to independently provide support services to the translational research program of the Pulmonary Vascular Biology Laboratory within the Critical Care Medicine and Pulmonary Branch (CCMPB) at the National Heart Lung and Blood Institute (NHLBI). This is a full-time on-site opportunity in Bethesda MD.

• Independently maintain human cells in culture as well as perform and assist with various experiments and molecular biology techniques and procedures
• Perform RNA and protein isolation from cell lysates
• Perform quantitative RT-PCR and Western blotting
• Perform serum plasma and PBMC preparation from whole blood for cryopreservation
• Use database for clinical sample tracking
• Maintain electronic laboratory notebook with detailed accounts of reagents and procedures used in experimental work
• In cases of new experimental designs locate the appropriate references and then set up apply and evaluate the procedure in question
• Collect organize analyze and interpret data. Prepare technical reports summaries protocols standard operating procedures and inventory updates
• Present data to principal investigator supervisors lab members and collaborators
• Maintain laboratory supply inventories including procurement of laboratory supplies reagents and equipment
• Oversee general lab maintenance and organization including equipment maintenance
• Troubleshoot problems with equipment or experimental techniques and develop methods to resolve these difficulties under the direction of the task leader
• Maintain laboratory safety and update records of safety trainings
• Operate and communicate effectively in a group team-oriented working environment
• Comply with institutions rules and regulations such as laboratory safety and waste disposal methods institutional review board protocols environmental health and safety

What You Will Need:

• PhD in Biology or related discipline such as Molecular and Cell Biology Immunology Biochemistry or a related field with a Minimum of THREE (3) of relevant experience in related fields such as molecular biology cell biology vascular biology or immunology OR a masters degree in biology or related discipline such as Molecular and Cell Biology Immunology Biochemistry or a related field with at least FIVE (5) years of relevant experience in related fields such as molecular biology cell biology vascular biology or immunology in lieu of PhD.
• Ability to independently perform RNA isolation and purification quantitative RT-PCR protein isolation (whole cell as well as nuclear and cytoplasmic fractionation) ELISA based assays Western blotting or light microscopy is required
• Experience with Microsoft 365 required
• Must be organized with a strong ability to multi-task prioritize and attentive to detail
• Must possess a high level of independent judgment and accuracy
• Needs to be able to obtain NIH public trust clearance

What Would Be Nice To Have:

• Able to independently and proficiently perform cell culture of human cell lines and/or primary human cells is required. Prior experience culturing endothelial cells is preferred
• Proficiency with flow cytometry and/or confocal microscopy is preferred
• Knowledge of proper laboratory disposal methods and safety procedures
• Experience with graphing software (e.g. GraphPad) preferred
• Ability to make recommendations to effectively resolve issues by using judgment that is consistent with standards practices policies procedures and regulations or law
• Strong interpersonal skills and an ability to establish and maintain effective working relationships with colleagues and collaborators
• Ability to work both independently and in a team-oriented environment
• Excellent oral and written communications skills including the ability to analyze scientific data and present to groups for peer review

What We Offer:

Guidehouse offers a comprehensive total rewards package that includes competitive compensation and a flexible benefits package that reflects our commitment to creating a diverse and supportive workplace.

Benefits include:

• Medical Rx Dental & Vision Insurance

• Personal and Family Sick Time & Company Paid Holidays

• Parental Leave

• 401(k) Retirement Plan

• Group Term Life and Travel Assistance

• Voluntary Life and AD&D Insurance

• Health Savings Account Health Care & Dependent Care Flexible Spending Accounts

• Transit and Parking Commuter Benefits

• Short-Term & Long-Term Disability

• Tuition Reimbursement Personal Development Certifications & Learning Opportunities

• Employee Referral Program

• Corporate Sponsored Events & Community Outreach

• annual membership

• Employee Assistance Program

• Supplemental Benefits via Corestream (Critical Care Hospital Indemnity Accident Insurance Legal Assistance and ID theft protection etc.)

• Position may be eligible for a discretionary variable incentive bonus

About Guidehouse

Guidehouse is an Equal Opportunity EmployerProtected Veterans Individuals with Disabilities or any other basis protected by law ordinance or regulation.

Guidehouse will consider for employment qualified applicants with criminal histories in a manner consistent with the requirements of applicable law or ordinance including the Fair Chance Ordinance of Los Angeles and San Francisco.

If you have visited our website for information about employment opportunities or to apply for a position and you require an accommodation please contact Guidehouse Recruiting at 1- or via email at . All information you provide will be kept confidential and will be used only to the extent required to provide needed reasonable accommodation.

All communication regarding recruitment for a Guidehouse position will be sent from Guidehouse email domains including @ or . Correspondence received by an applicant from any other domain should be considered unauthorized and will not be honored by Guidehouse. Note that Guidehouse will never charge a fee or require a money transfer at any stage of the recruitment process and does not collect fees from educational institutions for participation in a recruitment event. Never provide your banking information to a third party purporting to need that information to proceed in the hiring process.

If any person or organization demands money related to a job opportunity with Guidehouse please report the matter to Guidehouses Ethics Hotline. If you want to check the validity of correspondence you have received please contact . Guidehouse is not responsible for losses incurred (monetary or otherwise) from an applicants dealings with unauthorized third parties.

Guidehouse does not accept unsolicited resumes through or from search firms or staffing agencies. All unsolicited resumes will be considered the property of Guidehouse and Guidehouse will not be obligated to pay a placement fee.