Lab Technician

Benefits:

• 100% Medical Dental & Vision Coverage for Employees
• Paid Time Off (Including Holidays)
• Employee Referral Bonus
• 401K Match
• Tuition reimbursement and professional development opportunities
• Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)

Axle is seeking a Lab Technician to join our vibrant team at the National Institutes of Health (NIH) supporting the National Heart Lung and Blood Institute (NHLBI) located onsite inBethesda MD.

Qualificiations:

• Doctor of Philosophy degree (PhD) in Biology Molecular and Cell Biology Immunology Biochemistry or a related field
• At least three (3) years of related experience in the field(s) of molecular biology cell biology vascular biology immunology or a related field
• Able to independently and proficiently perform cell culture of human cell lines and/or primary human cells is required. Prior experience culturing endothelial cells is preferred
• Ability to independently perform RNA isolation and purification quantitative RT-PCR protein isolation (whole cell as well as nuclear and cytoplasmic fractionation) ELISA based assays Western blotting and light microscopy is required
• Proficiency with flow cytometry and/or confocal microscopy is preferred
• Knowledge of proper laboratory disposal methods and safety procedures
• Experience with Microsoft 365 required. Experience with graphing software (e.g. GraphPad) preferred
• Must be organized with a strong ability to multi-task prioritize and attentive todetail
• Must possess a high level of independent judgment andaccuracy
• Ability to make recommendations to effectively resolve issues by using judgment that is consistentwith standards practices policies procedures and regulations orlaw
• Strong interpersonal skills and an ability to establish and maintain effective working relationships with colleagues and collaborators
• Ability to work both independently and in a team-oriented environment
• Excellent oral and written communications skills including the ability to analyze scientific data and present to groups for peer review

Requirements:

• Independently maintain human cells in culture as well as perform and assist with various experiments and molecular biology techniques and procedures
• Perform RNA and protein isolation from cell lysates
• Perform quantitative RT-PCR and Western blotting
• Perform serum plasma and PBMC preparation from whole blood for cryopreservation
• Use database for clinical sample tracking
• Maintain electronic laboratory notebook with detailed accounts of reagents and procedures used in experimental work
• In cases of new experimental designs locate the appropriate references and then set up apply and evaluate the procedure in question
• Collect organize analyze and interpret data. Prepare technical reports summaries protocols standard operating procedures and inventory updates
• Present data to principal investigator supervisors lab members and collaborators
• Maintain laboratory supply inventories including procurement of laboratory supplies reagents and equipment
• Oversee general lab maintenance and organization including equipment maintenance
• Troubleshoot problems with equipment or experimental techniques and develop methods to resolve these difficulties under the direction of the task leader
• Maintain laboratory safety and update records of safety trainings
• Operate and communicate effectively in a group team-oriented working environment
• Comply with institutions rules and regulations such as laboratory safety and waste disposal methods institutional review board protocols environmental health and safety

The diversity of Axles employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment-based age race gender religion national origin disability marital status covered veteran status sexual orientation status with respect to public assistance and other characteristics protected under state federal or local law and to deter those who aid abet or induce discrimination or coerce others to discriminate.

Accessibility: If you need an accommodation as part of the employment process please contact:

Disclaimer: The above description is meant to illustrate the general nature of work and level of effort being performed by individuals assigned to this position or job description. This is not restricted as a complete list of all skills responsibilities duties and/or assignments required. Individuals may be required to perform duties outside of their position job description or responsibilities as needed.

This role has a market-competitive salary with an anticipated base compensation range listed below. Actual salaries will vary depending on a candidates experience qualifications skills and location.

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