Core Research Facility Technician III
Core Research Facility Technician III
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$ 45000 - 46000
1 Vacancy
Job Description
Core Research Facility Technician III
This position is a fulltime timelimited Core Research Facility Technician III (CRFT III) position in the Monoclonal Antibody (MA) Core in the Interdisciplinary Center for Biotechnology Research (ICBR). The MA Core provides scientific resources for the production purification and applications of monoclonal and polyclonal antibodies. The CRFT IIII will be involved in all aspects of monoclonal antibody production in mice including customer consultations rodent immunization and handling ELISAs mammalian cell culture and protein affinity chromatography. Additional responsibilities include the maintenance and operation of laboratory equipment such as microscopes centrifuges and microplate readers and cryogenic freezers. The incumbent must be detailoriented and customer service oriented with excellent verbal and written communication skills. Proficiency in MS Office Suite is desired with the ability to follow oral and written instructions/protocols and effective time management and planning.
- Perform affinity chromatography for monoclonal/polyclonal antibody purification. Develop and optimize purification methods. Analyze quality quantity and activity of purified antibody. Aliquot sterile culture media and chemical reagents. Culture and maintain hybridoma and recombinant antibody cell lines.
- Develop and perform biochemical assays (e.g. ELISA and western blot). Analyze experimental data. Keep legible accurate and complete records.
- Rodent handling and nonsurgical procedures for the purpose of generating unique antibodyproducing hybridoma cell lines.
- Serve as a contact for REES equipment alarms after hours weekends and holidays including troubleshooting equipment malfunctions during nonbusiness hours. Perform routine lab maintenance maintaining cryogenic freezers moving liquid nitrogen 300 lbs. and carbon dioxide 145 lbs. cylinders autoclaving waste washing labware preparing lab solutions and care and decontamination of cell culture incubators.
- Plan organize and carry out assigned lab and administrative tasks. Present data and generate electronic notes during lab meetings. Manage projects and communicate frequently with each client. Enter service requests using the CrossLab (iLab) system. Perform intake functions such as receiving and logging samples or requests for services. Ensure that timely progress is made on all assigned projects.
- Maintain information on each new hybridoma cell line and storage locations using an electronic inventory system. Periodically purge uncloned hybridoma cell lines from cryostorage.
- Other duties assigned such as maintaining chemical inventory and managing chemical waste pickup. Complete monthly hazardous waste checklist. Acting instructor of Mammalian Cell Culture Workshop.
$45000 $46000 per year commensurate with education and experience
In addition to your salary the University of Florida (UF) offers low cost State Health plans a number of Dental plans to fit you and your familys needs and Vision. Domestic partner coverage through GatorCare is also available. Optional plans such as life disability legal and accident insurance are also available. UF provides a variety of leave programs including vacation sick leave 11 paid holidays personal leave days and family medical leave. TEAMS employees accrue approximately 22 vacation days annually 13 sick days annually and 4 personal leave days typically used during the holiday closing period (accruals based on fulltime employment). UF also provides you the flexibility to deal with lifes challenges by offering paid family leave eight full weeks of leave over a 24month period. Build your retirement roadmap with competitive pension plans investment accounts and a host of voluntary addons such as 457 deferred compensation and 403(b) plans. UF Employees are also eligible for the GatorPerks discount program which provides big savings at various business and retailers!
BA/BS in related scientific field required with handson experience in basic laboratory and scientific methods. Optionally 4 years of experience in science related field with at least 3 years in core specific applications. Researchbased experience preferred. Core or technology specific licensure and/or certifications where applicable. Additionally yearly certificate and compliance training if required.
Researchbased experience in rodent handling mammalian tissue and cell culture ELISA and Western blotting and other basic chemistry and biochemistry laboratory method skills
In order to be considered you must upload your cover letter and resume.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.
Required Experience:
IC
Employment Type
Full-Time
