Intermediate Level Research Services Professional

University of Colorado Anschutz Medical Campus

Department:Barbara Davis Center for Diabetes

Job Title: Intermediate Level Research Services Professional

Position #: Requisition #:36604

Job Summary:

The Wortham Lab at the Barbara Davis Center for Diabetes is recruiting a professional research assistant to support our studies of pancreatic beta cell adaptation and failure in diabetes. Our laboratory studies fundamental processes related to how pancreatic beta cells sense and respond to stress. We employ a variety of techniques including genomics metabolomics genetics and physiology using both animal models of diabetes as well as primary culture of human pancreatic tissue. Under general supervision the incumbent will assist general studies in the laboratory using routine assays in biochemistry cell biology molecular biology and in vivo physiology. The incumbent will also be involved in general laboratory maintenance including ordering/restocking equipment maintenance and overseeing laboratory safety.

Key Responsibilities / Duties:

Animal work 40

• Tagging and genotyping of mice
• Assist with metabolic studies in mice
• Dissect and process mouse tissues for immunohistochemical analysis

Routine and advanced molecular biology biochemistry and cell biology techniques 40

• Standard bench work (e.g. ELISA PCR and RTPCR DNA and RNA purification protein extraction and western blotting tissue culture immunofluorescent staining and confocal microscopy)
• Culture cell lines and primary tissue
• Optimize and establish advanced molecular biology techniques including single cell genomics for analysis of gene expression and chromatin state

General lab duties 20

• Take inventory of laboratory supplies and order new supplies as necessary
• Equipment setup maintenance and repair
• Manage chemical inventories and arrange pickup of chemical waste
• Act as laboratory safety coordinator
• Assist and train junior team members

Work Location:

Onsite this role is expected to work onsite and is located in Aurora CO.

Why Join Us:

The Barbara Davis Center for Diabetes is a rich training environment that brings together basic science laboratories addressing pancreatic and immune defects in diabetes as well as clinical investigators directly testing new therapies for people with diabetes. Our basic research in pancreatic beta cell biology will interface with research across the Barbara Davis Center and the incumbent will have the opportunity to learn about all levels of diabetes researchbasic applied and clinical. Our work will contribute to a better understanding of human metabolism and has potential to lay the foundation for clinical studies improving the lives of people with diabetes. TheUniversity of Colorado Anschutz Medical Campus is the largest academic health center in the Rocky Mountain region and hosts world class laboratories across disciplines as well as stateoftheart core facilities. Professional research assistants in the laboratory will have the opportunity to gain technical expertise and develop professional skillsets beneficial to achieving a variety of longterm career goals.

Why work for the University

We haveAMAZINGbenefits and offerexceptional amounts of holiday vacation and sick leave! The University of Colorado offers an excellent benefits package including:

• Medical: Multiple plan options
• Dental: Multiple plan options
• Additional Insurance: Disability Life Vision
• Retirement 401(a) Plan: Employer contributes 10 of your gross pay
• Paid Time Off: Accruals over the year
• Vacation Days: 22/year (maximum accrual 352 hours)
• Sick Days: 15/year (unlimited maximum accrual)
• Holiday Days: 10/year
• Tuition Benefit: Employees have access to this benefit on all CU campuses
• ECO Pass: Reduced rate RTD Bus and light rail service

There are many additional perks & programs with the CU Advantage.

Equal Employment Opportunity Statement:

The University of Colorado (CU) is an Equal Opportunity Employer and complies with all applicable federal state and local laws governing nondiscrimination in employment. We are committed to creating a workplacewhere all individuals are treated with respect and dignity andwe encourage individuals from all backgrounds to apply.

Qualifications:

Minimum Qualifications:

• Bachelors degree in biology biochemistry physiology molecular biology genetics or related field
• One 1 year of experience in an experimental or wet laboratory

Conditions of Employment:

• Must be willing and able to work with live mice
• Must be in commuting distance or willing to relocate to commuting distance to Aurora CO.

A combination of education and related technical/paraprofessional experience may be substituted for the bachelors degree on a year for year basis.

Applicants must meet minimum qualifications at the time of hire.

Preferred Qualifications:

• Masters degree in biology biochemistry physiology molecular biology genetics or related field
• Two 2 years of experience in an experimental or wet laboratory
• Prior experience working with animal models such as mice including:
  • Experience in husbandry including breeding identification and genotyping
  • Knowledge of mammalian anatomy
  • Experience with surgical procedures including collecting tissue samples
• Experience with immunohistochemical techniques
• Experience with confocal microscopy and image acquisition
• Experience performing standard and routine laboratory techniques such as but not limited to:
  • Biochemical assays tissue culture ELISA DNA and RNA extraction and quantification polymerase chain reaction (PCR) protein extraction and western blotting

Knowledge Skills and Abilities:

• Knowledge of basic laboratory techniques including safety procedures and techniques
• Ability to communicate effectively both in writing and orally
• Ability to establish and maintain effective working relationships with employees at all levels throughout the institution
• Knowledge of basic molecular biology biochemistry cell biology and/or physiology
• Ability to interpret and master complex research protocol information
• Ability to work with small animal models
• Ability to keep organized and accurate laboratory records accurately recording workflows and data into electronic laboratory notebooks
• Knowledge of proper use and maintenance of laboratory equipment
• Ability to follow verbal and/or written instructions
• Ability to work with little supervision
• Strong organizational skills
• Detailoriented
• Ability to begin where others have left off on partially completed procedures
• Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills research methods and scientific principles.

How to Apply:

For full consideration please submit the following document(s):

1. A letter of interest describing relevant job experiences as they relate to listed job qualifications and interest in the position

2. Curriculum vitae / Resume

3. Three to five professional references including name address phone number (mobile number if appropriate) and email address

Questions should be directed to: Matthew Wortham

Screening of Applications Begins:

Immediately and continues.

Anticipated Pay Range:

The starting salary range or hiring range for this position has been established as HIRING RANGE $50357$64054

The above salary range or hiring range represents the Universitys good faith and reasonable estimate of the range of possible compensation at the time of posting. This position may be eligible for overtime compensation depending on the level.

Your total compensation goes beyond the number on your paycheck. The University of Colorado provides generous leave health plans and retirement contributions that add to your bottom line.

Total Compensation Calculator: Statement:

The University will provide reasonable accommodations to applicants with disabilities throughout the employment application process. To request an accommodation pursuant to the Americans with Disabilities Act please contact the Human Resources ADA Coordinator at .

Background Check Statement:

The University of Colorado Anschutz Medical Campus is dedicated to ensuring a safe and secure environment for our faculty staff students and visitors. To assist in achieving that goal we conduct background investigations for all prospective employees.

Vaccination Statement:

CU Anschutz strongly encourages vaccination against the COVID19 virus and othervaccine preventable diseases. If you work visit or volunteer in healthcare facilities or clinics operated by our affiliated hospital or clinical partners or by CU Anschutz you will be required to comply with the vaccination and medical surveillance policies of the facilities or clinics where you work visit or volunteer respectively. In addition if you work in certain research areas or perform certain safety sensitive job duties you must enroll in theoccupational health medical surveillance program.